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Rare-earth-incorporated polymeric vector for enhanced gene delivery

机译:掺入稀土的聚合载体可增强基因传递

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摘要

Cationic polymer PEI-CyD is doped with Nd by plasma technology to produce the gene vector: Nd@PEI-CyD. Luciferase expression and EGFP transfection experiments performed invitro reveal that Nd@PEI-CyD has significantly higher transfection efficiency than lipofectamine 2000 and PEI-CyD and the mechanism is studied and proposed. The rare-earth element, Nd, stimulates the energy metabolism of cells, enhances cell uptake of complexes/pDNA, and regulates the cellular pathways. These special features suggest a new strategy involving metal-incorporated non-viral gene vectors.
机译:通过等离子体技术将阳离子聚合物PEI-CyD掺入Nd,以产生基因载体:Nd @ PEI-CyD。体外进行的荧光素酶表达和EGFP转染实验表明,Nd @ PEI-CyD的转染效率明显高于lipofectamine 2000和PEI-CyD,并研究了其机理。稀土元素Nd刺激细胞的能量代谢,增强复合物/ pDNA的细胞摄取,并调节细胞途径。这些特殊功能提出了一种新的策略,涉及金属结合的非病毒基因载体。

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