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首页> 外文期刊>Biomaterials >Transplantation of allogenic chondrocytes with chitosan hydrogel-demineralized bone matrix hybrid scaffold to repair rabbit cartilage injury
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Transplantation of allogenic chondrocytes with chitosan hydrogel-demineralized bone matrix hybrid scaffold to repair rabbit cartilage injury

机译：壳聚糖水凝胶脱矿骨基质混合支架移植同种异体软骨细胞修复兔软骨损伤

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Cartilage tissue engineering is the hotspot of cartilage repair. The allogenic chondrocytes appear to be a promising source of seed cells in cartilage tissue engineering. In this study, we aimed to transplant allogenic chondrocytes with chitosan hydrogel (CS)-demineralized bone matrix (DBM) hybrid scaffold (CS/DBM) to repair rabbit cartilage injury with one-step operation. After the CS/DBM scaffold was successfully fabricated, it showed that the porous CS filled the large pores of DBM, which improved the distribution of seed cells in the CS/DBM scaffold. The allogenic chondrocytes at second passage were transplanted with different scaffolds to repair rabbit cartilage injury. Twenty-four weeks after surgery, the cartilage defect in the CS/DBM group was successfully filled as shown by MRI. Moreover, the histological score of CS/DBM group was significantly higher than that of the other groups. On the aspect of biomechanical property, the regenerated cartilage in the CS/DBM group were superior to those in the other groups as determined by nanoindentation. Meanwhile, no obvious inflammatory response was observed after the transplantation of allogenic chondrocytes at 24 weeks post-surgery. Furtherly, gene expression profile for cells within the repair tissue was compared with the allogenic chondrocytes before transplantation using Agilent microarray and RT-qPCR. The results showed that some genes beneficial to cartilage regeneration, such as BMP-7, HGF, and IGF-1, were upregulated one month after transplantation. Consequently, our study demonstrated that the transplantation of allogenic chondrocytes with CS/DBM scaffold successfully repaired rabbit cartilage injury with only one-step operation, thereby providing new insights into cartilage tissue engineering. (C) 2016 Elsevier Ltd. All rights reserved.
机译：软骨组织工程是软骨修复的热点。在软骨组织工程中，同种异体软骨细胞似乎是有希望的种子细胞来源。在这项研究中，我们的目标是将异体软骨细胞与壳聚糖水凝胶（CS）-脱矿骨基质（DBM）混合支架（CS / DBM）一起移植，以一步操作修复兔软骨损伤。成功制备CS / DBM支架后，表明多孔CS填充了DBM的大孔，从而改善了CS / DBM支架中种子细胞的分布。第二代同种异体软骨细胞移植有不同的支架以修复兔软骨损伤。 MRI显示，术后24周，CS / DBM组的软骨缺损被成功填充。此外，CS / DBM组的组织学评分明显高于其他组。在生物力学性能方面，通过纳米压痕测定，CS / DBM组的再生软骨优于其他组。同时，在术后24周移植同种异体软骨细胞后，未观察到明显的炎症反应。此外，使用安捷伦微阵列和RT-qPCR将移植前修复组织内细胞的基因表达谱与同种异体软骨细胞进行了比较。结果表明，移植后一个月，一些有益于软骨再生的基因如BMP-7，HGF和IGF-1被上调。因此，我们的研究表明，采用CS / DBM支架进行同种异体软骨细胞移植仅需一步操作即可成功修复兔软骨损伤，从而为软骨组织工程学提供新的见识。 （C）2016 Elsevier Ltd.保留所有权利。

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