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首页> 外文期刊>Brain research >Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo
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Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

机译：可生物降解的微球体局部释放神经营养因子可增强体内外运动和感觉轴突再生

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摘要

Neurotrophic factors (NTFs) promote nerve regeneration and neuronal survival after peripheral nerve injury. However, drawbacks related with administration and bioactivity during long periods limit their therapeutic application. In this study, PLGA microspheres (MPs) were used to locally release different NTFs and evaluate whether they accelerate axonal regeneration in comparison with free NTFs or controls. ELISA, SEM, UV/visible light microscopy, organotypic cultures of DRG explants and spinal cord slices were used to characterize MP properties and the bioactivity of the released NTFs. Results of organotypic cultures showed that encapsulated NTFs maintain longer bioactivity and enhance neurite regeneration of both sensory and motor neurons compared with free NTFs. For in vivo assays, the rat sciatic nerve was transected and repaired with a silicone tube filled with collagen gel or collagen mixed with PBS encapsulated MPs (control groups) and with free or encapsulated NGF, BDNF, GDNF or FGF-2. After 20 days, a retrotracer was applied to the regenerated nerve to quantify motor and sensory axonal regeneration. NTF encapsulation in MPs improved regeneration of both motor and sensory axons, as evidenced by increased numbers of retrolabeled neurons. Hence, our results show that slow release of NTFs with PLGA MP enhance nerve regeneration. (C) 2016 Elsevier B.V. All rights reserved.
机译：神经营养因子（NTFs）促进周围神经损伤后的神经再生和神经元存活。但是，与长期给药和生物活性有关的缺点限制了它们的治疗应用。在这项研究中，PLGA微球（MPs）用于局部释放不同的NTF，并评估它们与游离NTF或对照相比是否促进轴突再生。 ELISA，SEM，UV /可见光显微镜，DRG外植体的器官型培养和脊髓切片用于表征MP特性和释放的NTF的生物活性。器官型培养的结果表明，与游离NTF相比，封装的NTF保持更长的生物活性并增强感觉神经和运动神经元的神经突再生。对于体内测定，将大鼠坐骨神经横断并用硅胶管修复，该硅胶管装有胶原蛋白凝胶或胶原蛋白，并与PBS封装的MP（对照组）以及游离或封装的NGF，BDNF，GDNF或FGF-2混合。 20天后，将逆向示踪剂应用于再生神经，以量化运动和感觉轴突再生。 MPs中的NTF封装改善了运动轴突和感觉轴突的再生，这由增加后标记神经元的数量所证明。因此，我们的结果表明，用PLGA MP缓慢释放NTF可以增强神经再生。 （C）2016 Elsevier B.V.保留所有权利。

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