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首页> 外文期刊>Chemistry: A European journal >Palladium(II)-mediated assembly of biotinylated ion channels
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Palladium(II)-mediated assembly of biotinylated ion channels

机译：钯（II）介导的生物素化离子通道组装

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Simple synthetic methodology has been used to create biotinylated pyridyl cholate lipids that can undergo multiple self-assembly events when inserted into phospholipid vesicles; PdII links cholates into transmembrane lipids, while avidin laterally clusters these complexes together and concomitantly assembles the vesicles into aggregates. The transmembrane assembly of cholates by PdII "opened" the ion channels, whereas avidin addition produced vesicle aggregates, giving a system that mimicked both transmembrane transport and cellular adhesion. Complexation of these Pd ~(II)-linked cholates by avidin gave a measurable decrease in ion flow, suggesting some channels became blocked or were prevented from adopting the optimum geometry for ion conduction. This reflects the importance of spatially appropriate preorganisation when generating active supramolecular assemblies. Half is not better than the hole: Functionalization of pyridyl-cholate lipids with biotin gave "sticky" palladium(II)-gated ion channels that also mediated vesicle-vesicle adhesion. Several levels of self-assembly were involved in the functional system: Pd~(II)-mediated transmembrane assembly of the lipids, avidin-induced intramembrane clustering of lipids (black arrow), and intermembrane adhesion (white arrow) into vesicle aggregates (top).
机译：已经使用简单的合成方法来创建生物素化的吡啶基胆酸盐脂质，当将其插入磷脂囊泡时会经历多次自组装事件。 PdII将胆酸盐连接成跨膜脂质，而抗生物素蛋白将这些复合物横向聚集在一起，并随之将囊泡组装成聚集体。 PdII通过胆酸盐的跨膜组装“打开”了离子通道，而抗生物素蛋白的添加产生了囊泡聚集体，从而提供了一个既能跨膜运输又能与细胞粘附的系统。这些Pd〜（II）连接的胆酸盐与抗生物素蛋白的络合使离子流显着减少，表明某些通道被堵塞或无法采用最佳的几何结构进行离子传导。这反映了在生成活性超分子组装体时适当进行空间重组的重要性。一半不比孔好：用生物素官能化吡啶基-胆酸盐脂质会产生“粘性”的钯（II）门控离子通道，该通道也介导了囊泡与囊泡的粘附。功能系统涉及多个水平的自组装：Pd〜（II）介导的脂质跨膜组装，亲和素诱导的脂质膜内聚集（黑色箭头）和膜间粘附（白色箭头）进入囊泡聚集体（顶部） ）。

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