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首页> 外文期刊>Chemistry: A European journal >Fluorescent Labeling Agents for Quorum-Sensing Receptors (FLAQS) in Live Cells
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Fluorescent Labeling Agents for Quorum-Sensing Receptors (FLAQS) in Live Cells

机译：活细胞中群体感应受体（FLAQS）的荧光标记剂

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摘要

Live-cell fluorescent labeling of bacterial cell-to-cell communication pathways has emerged as a challenging problem. The use of labeled ligands with a fluorescent tag represents the simplest method of choice. However, such approaches are often accompanied by insufficient binding affinity towards the receptor and competition problems with the natural agonists. Herein, the first example of a fluorescently labeled bacterial quorum-sensing signaling compound is presented. The synthetic labeling agent mimics the activity of the natural agonist throughout a large concentration range, and excellent labeling of bacterial quorum-sensing receptors in live cells was achieved. This operationally simple, fast, and inexpensive method was successfully applied to the selective labeling of the Burkholderia cenocepacia quorumsensing receptor CepR. Furthermore, selective labeling was achieved in mixed bacterial cultures, demonstrating the potential of this approach as a very powerful tool to visualize quorum sensing in bacteria in their natural habitat.
机译：细菌细胞间通信途径的活细胞荧光标记已成为一个具有挑战性的问题。具有荧光标签的标记配体的使用代表了最简单的选择方法。然而，这些方法常常伴随着对受体的结合亲和力不足以及与天然激动剂的竞争问题。在此，提出了荧光标记的细菌群体感应信号化合物的第一个例子。合成标记剂可在较大浓度范围内模拟天然激动剂的活性，并且可以在活细胞中实现出色的细菌群体感应受体标记。这种操作简单，快速且廉价的方法已成功地应用于对伯克霍尔德菌新菌法定量受体CepR的选择性标记。此外，在混合细菌培养物中实现了选择性标记，证明了这种方法作为可视化自然栖息地细菌中群体感应的非常强大工具的潜力。

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