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Rationalising the Geometric Variation between the A and B Monomers in the 1.9 angstrom Crystal Structure of Photosystem II

机译:合理化光系统II 1.9埃晶体结构中A和B单体之间的几何变化

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摘要

Density functional theory calculations are reported on a set of models of the water-oxidising complex (WOC) of photosystem II (PSII), exploring structural features revealed in the most recent (1.9 angstrom resolution) X-ray crystallographic studies of PSII. Crucially, we find that the variation in the Mn-Mn distances seen between the A and B monomers of this crystal structure can be entirely accounted for, in the low oxidation state (LOS) paradigm, by consideration of the interplay between two hydrogen-bonding interactions involving proximate amino acid residues with the oxo bridges of the WOC, that is, His337 with O3 (which leads to a general elongation in the Mn-Mn distances between Mn1, Mn2 and Mn3) and Arg357 with O2 (which results in a specific elongation of the Mn2-Mn3 distance).
机译:在光系统II(PSII)的水氧化复合物(WOC)的一组模型上报道了密度泛函理论计算,探索了PSII的最新X射线晶体学研究(1.9埃分辨率)中揭示的结构特征。重要的是,我们发现,在低氧化态(LOS)范式中,考虑到两个氢键之间的相互作用,可以完全解释该晶体结构的A和B单体之间Mn-Mn距离的变化。邻近氨基酸残基与WOC的羰基桥的相互作用,即His337与O3(导致Mn1,Mn2和Mn3之间的Mn-Mn距离普遍延长)和Arg357与O2的相互作用(导致特定的Mn2-Mn3距离的延长)。

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