首页> 外文期刊>Acta Horticulturae >Selection of Castanea sativa Mill. for resistance to Phytophthora cinnamomi: testing of selected clones.
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Selection of Castanea sativa Mill. for resistance to Phytophthora cinnamomi: testing of selected clones.

机译:栗树磨的选择。对Phytophthora cinnamomi的抗性:选择克隆的测试。

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摘要

TRAGSA conducted an exhaustive prospecting of Castanea sativa trees resistant to ink disease in Galicia. These clones were established for in vitro propagation as well as tested for resistance. From the 206 selected trees, 199 were established in vitro with variable results. 69 clones were lost after establishment, 119 clones are already in stable culture and 88 are producing acclimated vitroplants regularly. Multiplication rate is low (between 0.4 and 1.5) but rooting rate varies from 34.4 to 100% with 67% of the clones showing rooting rates higher than 70%. Acclimation reaches 97% and acclimated plants are being used for the conduction of resistance tests and the establishment of field trials. As the adult excised stem test was not repetitive, acclimated vitroplants were root inoculated and the excised shoot test was conducted on rejuvenated vitroplant stems, to find out about the influence of the ontogenical age of the material. An in vitro test was also carried out and correlation between tests was determined. Clones P011, P013, P014, P042, C004, C005, C006 and C017 are classified as resistant by the substrate inoculation test, which we consider as the most reliable. However, only P014 and C004 showed resistance in the three different tests, while clones C006, C017 and P011, were considered resistant by two of the three tests. Resistant clones will be proposed to the National Catalogue of Base Materials.
机译:TRAGSA对加利西亚的抗墨迹病害的栗树进行了详尽的勘探。建立这些克隆以用于体外繁殖并进行抗性测试。从206棵选定的树中,体外建立了199棵,结果各异。建立后丢失了69个克隆,已经稳定培养了119个克隆,有88个正在定期生产适应环境的离体植物。繁殖率低(0.4到1.5之间),但生根率从34.4%到100%不等,其中67%的克隆的生根率高于70%。驯化率达到97%,并使用驯化的植物进行抗性测试和建立田间试验。由于成年的离体茎试验不是重复性的,因此要对适应化的离体植物进行根接种,然后对回生的离体植物茎进行离体芽试验,以了解该材料的个体发育年龄的影响。还进行了体外测试,并确定了测试之间的相关性。克隆接种试验将克隆P011,P013,P014,P042,C004,C005,C006和C017列为抗药性,我们认为这是最可靠的。但是,在三个不同的测试中,只有P014和C004表现出抗性,而在三个测试中的两个中,克隆C006,C017和P011被认为具有抗性。抗性克隆将被推荐到国家基础材料目录中。

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