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首页> 外文期刊>Acta Horticulturae >The infectivity of entomopathogenic fungi Beauvaria bassiana and Metarhizium anisopliae to Rhynchophorus ferrugineus (Olivier) stages under laboratory conditions.
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The infectivity of entomopathogenic fungi Beauvaria bassiana and Metarhizium anisopliae to Rhynchophorus ferrugineus (Olivier) stages under laboratory conditions.

机译：在实验室条件下，昆虫病原真菌 Beauvaria bassiana 和 Metarhizium anisopliae 对Rynchophorus ferrugineus （Olivier）阶段的感染性。

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Two entomopathogenic fungi, Beauvaria bassiana and Metarhizium anisopliae were evaluated for their pathogenicity against the larvae and adults of Rhynchophorus ferrugineus under laboratory conditions. Red palm weevil stages were collected from the infested palm trees, Al-Hasa Governorate, KSA, and reared on pieces of sugarcane. Three concentrations of each fungi spores were prepared, 1x107, 1x108 and 1x109 spores/ml. Adults of R. ferrugineus (male and female) and larvae were treated by dipping and injection bioassay techniques. The control treatment was treated with sterilized water. After treatments, insects were transferred to jars containing sugarcane. Insects were observed for three weeks and the percent of mortality recorded. The results showed variation in mortality for different stages. Mortality percentages were 40, 55, and 60 for male, female and larvae, respectively, at a concentration of 1x107 spore/ml of B. bassiana when insects were treated by dipping technique. Mortality reached 80, 85 and 85% for male, female and larvae, respectively when treated by injection at the same concentration. Mortality percentages were 35, 50, and 60 for male, female and larvae, respectively at a concentration of 1x107 spore/ml of M. anisopliae when insects were treated by dipping. The mortality reached 70, 60, and 70% for the previous stages when treated by injection at the same concentration after ten days of observation. The percent of mortality increased with increasing concentration of fungus spores. The percent of mortality reached 70 and 80 for female and male when contaminated males were transferred to jars containing untreated females after 17 days of transfer. The percent of mortality reached 10% in the control treatment during that period.
机译：在实验室条件下，对两种昆虫病原真菌 Beauvaria bassiana 和 Metarhizium anisopliae 进行了评价，以评价它们对鼠李和成虫的致病性。从受感染的棕榈树（哈萨克斯坦省哈萨克斯坦州）收集红棕象鼻虫阶段，并饲养在甘蔗片上。每种真菌孢子的三种浓度分别为1x10 7 ，1x10 8 和1x10 9 / ml。 R的成人用浸入和注射生物分析技术处理了ferrugineus（雄性和雌性）和幼虫。对照处理用无菌水处理。处理后，将昆虫转移到装有甘蔗的广口瓶中。观察昆虫三个星期，记录死亡率。结果显示不同阶段的死亡率差异。男性，女性和幼虫的死亡率分别为1x10 7 孢子/ ml B，分别为40、55和60。用浸蘸技术处理昆虫时的鲈鱼。当以相同浓度注射治疗时，男性，女性和幼虫的死亡率分别达到80％，85％和85％。男性，女性和幼虫的死亡率分别为1x10 7 孢子/ ml M，分别为35、50和60。用浸蘸法处理昆虫观察十天后，以相同浓度注射治疗，前一阶段的死亡率分别达到70％，60％和70％。死亡率百分数随真菌孢子浓度的增加而增加。当受污染的雄性在转移17天后转移到装有未处理雌性的广口瓶中时，雌性和雄性的死亡率分别达到70和80。在此期间，对照治疗的死亡率达到10％。

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