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首页> 外文期刊>Acta Horticulturae >Ploidy levels of degenerated embryos in the crosses between diploid and tetraploid cyclamen.
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Ploidy levels of degenerated embryos in the crosses between diploid and tetraploid cyclamen.

机译：二倍体和四倍体仙客来杂交的退化胚的倍性水平。

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摘要

In reciprocal crosses between diploid and tetraploid cyclamen, almost all zygotic embryos were degenerated. Ploidy levels of the degenerated embryos were, however, obscure. Therefore, embryo rescue by the ovule culture in the crosses were examined. A lot of progenies were obtained in the 2x x 4x crosses, when the ovule culture used medium with coconut water was done 28 days after pollination. Although almost all fruits were dropped 28 days after pollination in the 4x x 2x crosses, some progenies were obtained by the ovule culture at 7-28 days after pollination. Almost all progenies obtained by the ovule culture after the reciprocal crosses between the diploids and tetraploids were triploids, suggesting that many triploid zygotes were formed in the crosses. However, some tetraploid, pentaploid and hexaploid progenies were also obtained. Origin of the pentaploids was suggested the fertilization between a reduced gamete from a diploid plant and an unreduced gamete from a tetraploid plant. These results suggest that many triploid zygotes are formed in the crosses between diploid and tetraploid cyclamen, whereas development of the triploid embryo without the embryo rescue is inhibited. It also shows the possibility that pentaploids and hexaploids as well as triploids and tetraploids can be obtained in the crosses using the ovule culture, depending on the cross combination.
机译：在二倍体和四倍体仙客来之间的相互杂交中，几乎所有合子胚都退化了。然而，退化胚的倍性水平是模糊的。因此，研究了通过杂交胚珠培养对胚胎的拯救。当授粉后28天完成用椰子水培养的胚珠培养液时，在2×××4××杂交中获得了许多后代。尽管几乎所有的果实在授粉后28天掉落在4×x 2×i杂交中，但在授粉后7-28天通过胚珠培养获得了一些后代。在二倍体和四倍体之间相互杂交后，通过胚珠培养获得的几乎所有子代都是三倍体，这表明在杂交中形成了许多三倍体合子。但是，也获得了一些四倍体，五倍体和六倍体后代。五倍体的起源暗示了来自二倍体植物的减少的配子和来自四倍体植物的未减少的配子之间的受精。这些结果表明，在二倍体和四倍体仙客来之间的杂交中形成了许多三倍体合子，而三倍体胚的发育受到了胚胎的拯救而受到了抑制。这也表明，根据杂交组合，利用胚珠培养可以在杂交中获得五倍体和六倍体以及三倍体和四倍体。

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