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首页> 外文期刊>Acta Horticulturae >Solanum pennellii LA716 as a source of genes improving in vitro organogenesis in cultivated tomato.
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Solanum pennellii LA716 as a source of genes improving in vitro organogenesis in cultivated tomato.

机译:茄茄LA716是改善栽培番茄体外器官发生的基因来源。

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In the present work we are reporting the high in vitro regeneration capacity of the tomato related wild species S. pennellii LA716, which enabled us to used a collection of 50 introgression lines (ILs), each containing small chromosomal segments of LA716 introgressed and mapped into the cultivar 'M82'. We found a high shoot regeneration capacity for IL3-2, IL6-1, IL7-1 (7-2, 7-3), IL 8-3 (8-2), IL-9-1 (9-2) and IL10-2 (10-3), when 12-days-old cotyledon explants were cultivated in MS medium containing 5.0 micro M BAP. This means that S. pennellii probably presents superior alleles for in vitro regeneration in such chromosomal segments. Since ILs 3-2, 7-1, 8-3, and 10-2 also presented enhanced root formation in MS medium containing 0.4 micro M NAA, they may represent novel alleles controlling the competence to assume different cell fates, rather than the induction of a specific organ. The alleles discovered here will provide for the characterization and isolation of important genes for plant development studies and biotechnological applications.
机译:在当前的工作中,我们报告了与番茄相关的野生种S. pennellii LA716的高体外再生能力,这使我们能够使用50个基因渗入系(IL)的集合,每个渗入系包含LA716的小染色体区段,渗入并定位到品种“ M82”。我们发现IL3-2,IL6-1,IL7-1(7-2、7-3),IL 8-3(8-2),IL-9-1(9-2)和IL10-2(10-3),当在含有5.0 micro M BAP的MS培养基中培养12天大的子叶外植体时。这意味着半夏葡萄球菌可能在此类染色体片段中呈现出优越的等位基因,用于体外再生。由于ILs 3-2、7-1、8-3和10-2在含有0.4 micro M NAA的MS培养基中也表现出增强的根系形成,因此它们可能代表了控制等位基因假定而不是诱导细胞命运的新等位基因。特定器官。在这里发现的等位基因将为植物发育研究和生物技术应用提供重要基因的表征和分离。

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