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首页> 外文期刊>Acta Horticulturae >Physiological response to nitrogen deficiency stress of in vitro grown potato genotypes.
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Physiological response to nitrogen deficiency stress of in vitro grown potato genotypes.

机译:体外培养的马铃薯基因型对缺氮胁迫的生理响应。

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Increasing nutrient use efficiency is an important aspect in sustainable plant production in order to optimize the use of resources and minimize environmental pollution. Our investigations aim at the identification of genotypic differences which will allow analyzing specific components of nitrogen use efficiency in potato. In addition to characterizing a set of cultivars under field and greenhouse conditions an in vitro culture system with four different nitrogen levels was established in order to investigate if specific components of nitrogen use efficiency can be assessed under such conditions. For this purpose shoot tips were grown fixed in perforated stainless steel plates in 500 ml glas vessels with 50 ml liquid culture medium. The nitrogen levels of the media corresponded to full, 1/2, 1/4 and 1/8 of the original MS concentration, i.e., 60, 30, 15 and 7.5 mmol/L. Specific morphological parameters were recorded within three weeks of culture and nitrogen uptake and assimilation was analyzed. The vegetative development of plantlets proved to be differentially affected by a reduction of nitrogen in the culture medium and varied with the genotype. Furthermore, determination of nitrogen withdrawal from the culture media as well as analysis of crude protein content in shoots and roots allowed discrimination among genotypes with respect to nitrogen uptake and utilization capacity.
机译:为了优化资源利用并最小化环境污染,提高养分利用效率是可持续植物生产的重要方面。我们的研究旨在鉴定基因型差异,这将有助于分析马铃薯氮素利用效率的特定组成部分。除了表征田间和温室条件下的一组栽培品种外,还建立了具有四个不同氮水平的体外培养系统,以研究是否可以在这种条件下评估氮利用效率的特定组成部分。为此,将芽尖固定在带有50 ml液体培养基的500 ml玻璃容器中的带孔不锈钢板上。培养基中的氮含量分别相当于原始MS浓度的完全,1 / 2、1 / 4和1/8,即60、30、15和7.5 mmol / L。在培养的三周内记录特定的形态学参数,并分析氮吸收和同化。幼苗的营养发育被培养基中氮的减少不同地影响,并随基因型而变化。此外,测定从培养基中抽出的氮以及对枝条和根中粗蛋白含量的分析可以区分基因型之间的氮吸收和利用能力。

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