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首页> 外文期刊>Acta Horticulturae >Studies on the Mechanism of Seedless and Seedlessness in 'Kyoho' (Vitis vinifera L. x Vitis labruscana Bailey) Grapes Induced by Streptomycin
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Studies on the Mechanism of Seedless and Seedlessness in 'Kyoho' (Vitis vinifera L. x Vitis labruscana Bailey) Grapes Induced by Streptomycin

机译：链霉素诱导的'Kyoho'（Vitis vinifera L. x Vitis labruscana Bailey）葡萄无核和无核机理的研究

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The objective of this study was to investigate the mechanism of seedless induction by streptomycin in 'Kyoho' grapes. Inflorescences were dipped with 125 ppm streptomycin six days before flower bloom. Pollen grains of treated inflorescences were smaller and had lower germination percentage than those of untreated inflorescences. Pollen tube growth was inhibited and exhibited wavy tube structure and swelling of the tube tips in Brewbaker and Kwack (B&K) medium containing streptomycin. Pollen tube growth inside styles also exhibited the same characteristics. Ovules showed abnormalities after full bloom in the treated inflorescences but less expressive in mutual pollination flowers (i.e., emasculation and cross pollination between treated and untreatedflowers) indicating an inhibitory effect of this antibiotic on ovule development. Pollen germination and pollen tube growth of four stenospermocarpic seedless cultivars were also examined. The pollen tubes showed the similar characteristics as observedin the streptomycin treated 'Kyoho' grapes. The results suggested that wavy walls, swollen tips and callose plug deposition were common in seedless cultivars and streptomycin treated 'Kyoho' grapes. However, ovule development was also affected by streptomycin application because seedless percentage was reduced by mutual pollination.
机译：这项研究的目的是研究链霉素在“巨峰”葡萄中无核诱导的机理。在花朵盛开前六天，用125 ppm链霉素浸入花序。与未处理的花序相比，处理的花序的花粉粒更小并且发芽率更低。在含有链霉素的Brewbaker和Kwack（B＆K）培养基中，花粉管的生长受到抑制，并呈现出波浪状的管结构和管尖的膨胀。花粉管内部样式的生长也表现出相同的特征。在处理后的花序盛开后，胚珠显示异常，但在相互授粉的花中表达较少（即在处理过的和未处理的花之间有去雄和交叉授粉），表明该抗生素对胚珠发育有抑制作用。还研究了四个无角掌果无核品种的花粉萌发和花粉管生长。花粉管显示出与链霉素处理的“巨峰”葡萄相似的特性。结果表明，无核栽培品种和链霉素处理的“ Kyoho”葡萄常见有波纹状的壁，尖尖的肿胀和call质堵塞的沉积。但是，链霉素的使用也会影响胚珠的发育，因为无核的百分比会因相互授粉而减少。

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