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首页> 外文期刊>Acta Horticulturae >Callus and Plantlets Produced from In Vitro Culture of Anthers of Actinidia chinensis
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Callus and Plantlets Produced from In Vitro Culture of Anthers of Actinidia chinensis

机译：中华猕猴桃花药离体培养产生的愈伤组织和小植株

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Homozygous genotypes are valuable for genomic and genetic studies in higher plants, but obtaining homozygous perennial plants using conventional breeding technologies is challenging, with the long times and large field areas required for several back-crossed generations. It is especially difficult with Actinidia because of dioecy, long generation times, high heterozygosity and variation in ploidy from diploid to octoploid. In vitro androgenesis through anther or microspore culture offers a route to develop the haploid and dihaploid plants often used in modern crop breeding, genetics and genomic studies. I report in vitro culture of anthers of six genotypes of diploid Actinidia chinensis resulting in production of callus and regeneration of plantlets. Preliminary results showed that genotype, plant growth regulators, the stage of microspore development and pre-treatments (cold, heat shock) all affected callus formation. Confirmation of their callus origin, homozygosity and determination of the ploidy levels of plantlets are underway.
机译：纯合子的基因型对于高等植物的基因组和遗传研究很有价值，但是使用常规育种技术获得纯合的多年生植物是具有挑战性的，这需要几个回交世代的长时间和大领域。猕猴桃特别困难，因为其雌雄同体，长世代，高杂合度以及倍体从二倍体到八倍体的变化。通过花药或小孢子培养的体外雄激素形成提供了开发单倍体和二倍体植物的途径，这些植物通常用于现代作物育种，遗传学和基因组研究。我报告了六倍体中华猕猴桃六种基因型花药的体外培养，导致了愈伤组织的产生和幼苗的再生。初步结果表明，基因型，植物生长调节剂，小孢子发育阶段和预处理（冷，热休克）均影响愈伤组织的形成。他们的愈伤组织起源，纯合性的确认以及小植株的倍性水平的测定正在进行中。

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