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首页> 外文期刊>Acta Horticulturae >Diagnosis and Detection of Plum Pox Virus: State-of-the-Art and Future Options
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Diagnosis and Detection of Plum Pox Virus: State-of-the-Art and Future Options

机译：李子痘病毒的诊断和检测：最新技术和未来选择

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The control of Plum pox virus (PPV) and the management of sharka, require the use of reliable detection methods. Despite the considerable efforts made in many countries, PPV has been reported in most of the important Prunus growing countries worldwide. Moreover, it is occasionally intercepted in imported fruit-tree scion wood. Illegal trafficking and insufficiently controlled exchange of plant material are the main pathways of PPV spread over long distances. For decades, since the first description of sharka in 1932, there has been neither public awareness nor reliable methods applicable on a large-scale. ELISA technique reported for plant viruses in 1977 revolutionized PPV diagnosis to its advantage. Nevertheless, for more than a decade, ELISA techniques have been based exclusively on polyclonal antibodies that often present problems of specificity and other drawbacks. The production and use of specific monoclonal antibodies and later recombinant antibodies, constituted the second revolutionary fact in PPV detection and characterization. An example of their usefulness is that about ten million ELISAs for universal PPV detection have been performed using 5B-IVIA/AMR since 1994. The emergence of molecular amplitication methods revolutionized PPV detection, as well. The EPPO (2004) protocol for PPV is based on validated techniques and reagents. Nowadays, real-time RT-PCR techniques constitute the gold standard for PPV detection. Different formats including direct systems for sample preparation areincluded in the current IPPC-FAO (2012) protocol for PPV. The choice of the most appropriate detection method is crucial and must be related to the final goal or purpose of the analysis. The combination of ELISA-5B based and spot real-time RT-PCR techniques reaches 100% accuracy for PPV detection. "Next generation sequencing" is a powerful strategy for PPV detection and characterization and could be a potential substitute for biological indexing.
机译：控制李子痘病毒（PPV）和管理sharka，需要使用可靠的检测方法。尽管在许多国家做出了巨大的努力，但世界上大多数重要的李子种植国都报告了PPV。此外，它有时会被进口的果树接穗木拦截。 PPV的非法贩运和管制交换不足是PPV长距离传播的主要途径。自从1932年首次对sharka进行描述以来，几十年来，一直没有公众意识或可大规模应用的可靠方法。 1977年报道的针对植物病毒的ELISA技术彻底改变了PPV诊断的优势。然而，十多年来，ELISA技术一直仅基于多克隆抗体，而多克隆抗体经常会出现特异性和其他缺点。特异性单克隆抗体和后来的重组抗体的生产和使用，构成了PPV检测和鉴定的第二个革命性事实。自1994年以来，使用5B-IVIA / AMR已进行了约一千万次用于通用PPV检测的ELISA。分子扩增方法的出现也彻底改变了PPV的检测方法。 PPV的EPPO（2004）协议基于经过验证的技术和试剂。如今，实时RT-PCR技术已成为PPV检测的金标准。当前用于PPV的IPPC-FAO（2012）协议中包括不同格式，包括用于样品制备的直接系统。选择最合适的检测方法至关重要，并且必须与分析的最终目的或目的相关。基于ELISA-5B和现场实时RT-PCR技术的结合，PPV检测的准确性达到100％。 “下一代测序”是PPV检测和表征的强大策略，并且可能替代生物学索引。

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