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首页> 外文期刊>Acta Horticulturae >Ovule Culture and Embryo Rescue Facilitate Interspecific Hybridisation in Blueberry (Vaccinium spp.)
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Ovule Culture and Embryo Rescue Facilitate Interspecific Hybridisation in Blueberry (Vaccinium spp.)

机译：胚珠培养和胚胎抢救促进蓝莓的种间杂交（越橘属）

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摘要

About 80 blueberry (Vaccinium) accessions represent four species in New Zealand collections. They are highly heterogeneous and constitute a valuable resource as parental material for breeding. However, hybridisation between different species and ploidy levels does not produce viable seed in vivo. To increase the variation available to breeders, and to introgress characters from different species into parental breeding populations, interspecific, interploidy crosses were made. Ovules were cultured aseptically 14, 28 and 35 days after pollination. Embryos were dissected from the enlarged ovules and cultured on regeneration media. From fourteen of these crosses, more than 200 plants regenerated within 6-10 weeks. In ovulo embryo culture was more successful when the maternal parent had a lower ploidy than the male parent. Flow cytometry revealed that 4x x 6x crosses )roduced pentaploids, while the progeny of 4x x 5x and 5x x 6a: crosses had nuclear )NA contents similar to that of the maternal parent. Random Amplified Polymorphic DNA and Simple Sequence Repeat marker analyses are being used to confirm the hybrid nature of these progenies. Hybrid plants are being propagated in vitro, acclimated in the greenhouse and established in field trials. Our study has demonstrated that in vitro ovule culture and embryo rescue can be used to overcome post-zygotic barriers to interspecific hybridisation between Vaccinium species with different ploidy levels.
机译：新西兰收集物中约有80种蓝莓（Vaccinium）品种代表4种。它们是高度异质的，并且构成了作为育种亲本的宝贵资源。但是，不同物种和倍性水平之间的杂交不能在体内产生可行的种子。为了增加育种者可用的变异，并使不同物种的性状渗入亲本育种群体，进行了种间，倍性杂交。授粉后14、28和35天无菌培养胚珠。从扩大的胚珠切开胚，并在再生培养基上培养。从这些杂交中的十四个中，在6-10周内再生了200多个植物。在卵子中，当母本的倍性低于雄本时，胚胎培养会更成功。流式细胞仪显示4x x 6x杂交产生了五倍体，而4x x 5x和5x x 6a：杂交的后代的核NA含量与母本相似。随机扩增多态性DNA和简单序列重复标记分析已用于确认这些后代的杂交性质。杂交植物正在体外繁殖，在温室中适应并在田间试验中建立。我们的研究表明，体外胚珠培养和胚胎抢救可以用来克服合子后障碍，以不同倍性水平的越桔种之间进行种间杂交。

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