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Root system architecture in reciprocal grafts of apple rootstock-scion combinations

机译:苹果砧木-接穗组合互作的根系结构

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Many studies have demonstrated the effects of rootstocks on the growth and architecture of scions, but there has been comparatively little study of the physiological effects of scions on the performance of rootstocks. Three commercially available apple rootstocks conferring differing levels of vigour, M.27 (highly dwarfing), M.9 (dwarfing) and M.116 (semi-invigorating) were used as both rootstock and scion in reciprocally grafted combinations to determine the effect of the scion upon the rootstock and root system architecture (RSA). The trees were grown in thin rhizotrons (100 cm height, 30 cm width and 3 cm depth) containing loam and placed in a heated glasshouse with supplementary lighting and fertigation. The root systems were photographed with aDSLR camera at monthly intervals for a period of five months, and the images analysed using SmartRoot and MATLAB software to determine total root length and root diameter frequency. The height and stem diameter of the scions were also measured. Differences in total root length and root distribution were apparent after five months. M.27 had a shallow and fibrous root system whereas M.116 had a deep and coarse root system with more lateral roots appearing deeper in the soil. Our results show that the scion can have a significant effect that manifests as changes to the RSA, however, the extent of these changes are dependent upon the rootstock-scion combination.
机译:许多研究已经证明了砧木对接穗的生长和结构的影响,但是关于接穗对砧木性能的生理影响的研究相对较少。 M.27(高度矮化),M.9(矮度)和M.116(半活跃)三种赋予不同活力的苹果砧木被用作嫁接组合的砧木和接穗,以确定根茎和根系统架构(RSA)的接穗。树木在含有壤土的薄根茎(高100厘米,宽30厘米,深3厘米)中生长,并置于带有辅助照明和施肥的加热温室中。每月用aDSLR相机对根系照相,为期五个月,并使用SmartRoot和MATLAB软件分析图像以确定总根长和根直径频率。还测量了接穗的高度和茎直径。五个月后,总根长和根分布的差异明显。 M.27的根系较浅,呈纤维状,而M.116的根系较深而较粗,在土壤中更多的侧根出现在较深的地方。我们的结果表明,接穗可以具有明显的作用,表现为RSA的变化,但是,这些变化的程度取决于砧木-接穗的组合。

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