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首页> 外文期刊>ACS applied materials & interfaces >Ceria Doped Zinc Oxide Nanoflowers Enhanced Luminol-Based Electrochemiluminescence Immunosensor for Amyloid-beta Detection
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Ceria Doped Zinc Oxide Nanoflowers Enhanced Luminol-Based Electrochemiluminescence Immunosensor for Amyloid-beta Detection

机译：二氧化铈掺杂的氧化锌纳米花增强了基于鲁米诺的电化学发光免疫传感器的β-淀粉样蛋白检测。

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摘要

In this work, ceria doped ZnO nanomaterials with flower-structure (Ce:ZONFs) were prepared to construct a luminol-based electrochemiluminescence (ECL) immunosensor for amyloid-beta protein (A beta) detection. Herein, carboxyl groups ( COOH) covered Ce:ZONFs were synthesized by a green method with lysine as reductant. After that, Ce:ZONFs-based ECL nano composite was prepared by combining the luminophore of luminol and Ce:ZONFs via amidation and physical absorption. Luminol modified on Ce:ZONFs surface could generate a strong ECL signal under the assistance of reactive oxygen species (ROSs) (such as OH center dot and O-2(center dot-)), which were produced by a catalytic reaction between Ce:ZONFs and H2O2. It was worth noticing that a quick Ce4+ <-> Ce3+ reaction in this doped material could increase the rate of electron transfer to realize the signal amplification. Subsequently, the luminol functionalized Ce:ZONFs (Ce:ZONFs-Lum) were covered by secondary antibody (Ab(2)) and glucose oxidase (GOD), respectively, to construct a novel Ab(2) bioconjugate (Ab(2)-GOD@Ce:ZONFs-Lum). The wire-structured silver-cysteine complex (AgCys NWs) with a large number of -COOH, which was synthesized by AgNO3 and L-cysteine, was used as substrate of the immunosensor to capture the primary antibody (Ab(1)). Under the optimal conditions, this proposed ECL immunosensor had exhibited high sensitivity for A beta detection with a wide linear range from 80 fg/mL to 100 ng/mL and an ultralow detection limit of 52 fg/mL. Meanwhile, this biosensor had good specificity for A beta, indicating that the provided strategy had a promising potential in the detection of A beta.
机译：在这项工作中，制备了具有花结构（Ce：ZONFs）的二氧化铈掺杂的ZnO纳米材料，以构建用于淀粉样β蛋白（A beta）检测的基于鲁米诺的电化学发光（ECL）免疫传感器。在此，以赖氨酸为还原剂通过绿色方法合成了羧基（COOH）覆盖的Ce：ZONF。之后，通过酰胺化和物理吸收结合鲁米诺和Ce：ZONFs的发光体，制备了基于Ce：ZONFs的ECL纳米复合材料。在Ce：ZONFs表面改性的鲁米诺可以在活性氧（ROSs）（例如OH中心点和O-2（中心点））的协助下产生强ECL信号，这些活性氧是由Ce： ZONF和H2O2。值得注意的是，在该掺杂材料中快速的Ce4 +-Ce3 +反应可以提高电子转移速率，从而实现信号放大。随后，分别用二抗（Ab（2））和葡萄糖氧化酶（GOD）覆盖发光功能化的Ce：ZONFs（Ce：ZONFs-Lum），以构建新型Ab（2）生物缀合物（Ab（2）- GOD @ Ce：ZONFs-Lum）。由AgNO3和L-半胱氨酸合成的具有大量-COOH的线状银-半胱氨酸复合物（AgCys NWs）被用作免疫传感器的底物以捕获一抗（Ab（1））。在最佳条件下，这种拟议的ECL免疫传感器对Aβ检测具有很高的灵敏度，线性范围从80 fg / mL到100 ng / mL，检测极限为52 fg / mL。同时，该生物传感器对A beta具有良好的特异性，表明所提供的策略在A beta的检测中具有广阔的前景。

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