• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Determination of cardiac contractility in awake unsedated mice with a fluid-filled catheter.
【24h】

Determination of cardiac contractility in awake unsedated mice with a fluid-filled catheter.

机译：用充液导管测定清醒的未镇静小鼠的心脏收缩力。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Today, cardiac contractility in mice is exclusively measured under anesthesia or in sedated animals because the catheters available are too rigid to be used in awake mice. We therefore developed a new catheter (Pebax 03) to measure cardiac contractility in conscious mice. In this study, we evaluated the accuracy and utility of this new catheter for assessment of cardiac contractility in anesthetized and conscious mice. With the use of a balloon-pop test, the Pebax catheter with an inner diameter of 0.3 mm was found to exhibit a high natural frequency, a low damping coefficient, and a flat frequency of up to 50.5 +/- 0.6 Hz. Under anesthesia (0.5% or 1.0% halothane), no difference was found in heart rate (HR), left ventricular (LV) systolic pressure (LVSP), the maximum rates of LV pressure rise and fall (LV dP/dt(max) and LV dP/dt(min), respectively), ejection time (ET), and isovolumic relaxation time constant (tau) when measured with either the 1.4-Fr Millar or Pebax 03 catheter. However, when HR, LVSP, LV dP/dt(max), and LV dP/dt(min) were recorded with the Pebax catheter in awake mice, values were significantly higher, and ET and tau were lower, than under anesthesia, suggesting a major impact of anesthesia on these parameters. The Pebax catheter was also used in a normotensive one-renin gene mouse model of cardiac hypertrophy induced by DOCA and salt. In this model, DOCA-salt induced a severe decrease in cardiac contractility in the absence of changes in blood pressure. These data demonstrate that cardiac contractility can be measured very accurately in conscious mice. This new device can be of great help in the investigation of cardiac function in normal and genetically engineered mice.
机译：如今，由于可用的导管太硬而无法在清醒的小鼠中使用，因此仅在麻醉或镇静动物中测量小鼠的心脏收缩力。因此，我们开发了一种新导管（Pebax 03）来测量清醒小鼠的心脏收缩力。在这项研究中，我们评估了这种新型导管在麻醉和清醒小鼠中评估心脏收缩力的准确性和实用性。通过使用气球弹出测试，发现内径为0.3 mm的Pebax导管表现出较高的固有频率，较低的阻尼系数以及高达50.5 +/- 0.6 Hz的平坦频率。在麻醉（0.5％或1.0％氟烷）下，心率（HR），左心室（LV）收缩压（LVSP），LV压升高和降低的最大速率（LV dP / dt（max））均无差异当使用1.4-Fr Millar或Pebax 03导管测量时，分别为LV dP / dt（min）和LV dP / dt（min），射血时间（ET）和等容松弛时间常数（tau）。但是，当用清醒的小鼠用Pebax导管记录HR，LVSP，LV dP / dt（max）和LV dP / dt（min）时，其值明显高于麻醉下的值，而ET和tau值较低。麻醉对这些参数的重大影响。 Pebax导管还用于由DOCA和盐诱导的心脏肥大的血压正常的单肾素基因小鼠模型中。在该模型中，DOCA盐在血压无变化的情况下引起心脏收缩力严重降低。这些数据表明，在有意识的小鼠中可以非常准确地测量心脏收缩力。这项新设备在研究正常和基因工程小鼠的心脏功能方面可以提供很大帮助。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号