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首页> 外文期刊>American Journal of Physiology >Renal epithelial cells constitutively produce a protein that blocks adhesion of crystals to their surface.
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Renal epithelial cells constitutively produce a protein that blocks adhesion of crystals to their surface.

机译：肾上皮细胞组成性地产生一种蛋白质，该蛋白质可阻止晶体粘附于其表面。

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摘要

Attachment of newly formed crystals to renal tubular epithelial cells appears to be a critical step in the development of kidney stones. The present study was undertaken to identify autocrine factors released from renal epithelial cells into the culture medium that inhibit adhesion of calcium oxalate crystals to the cell surface. A 39-kDa glycoprotein that is constitutively secreted by renal cells was purified by gel filtration chromatography. Amino acid microsequencing revealed that it is novel and not structurally related to known inhibitors of calcium oxalate crystallization. Hence, it was named crystal adhesion inhibitor, or CAI. Immunoreactive CAI was detected in diverse rat tissues, including kidney, heart, pancreas, liver, and testis. Immunohistochemistry revealed that CAI is present in the renal cell cytosol and is also on the plasma membrane. Importantly, CAI is present in normal human urine, from which it can be purified using calcium oxalate monohydrate crystal affinity chromatography. CAI could be an important defense against crystal attachment to tubular cells and the subsequent development of renal stones in vivo.
机译：新形成的晶体附着在肾小管上皮细胞上似乎是肾结石发展的关键步骤。进行本研究以鉴定从肾上皮细胞释放到培养基中的自分泌因子，其抑制草酸钙晶体粘附到细胞表面。通过凝胶过滤色谱法纯化由肾细胞组成性分泌的39kDa糖蛋白。氨基酸微测序表明它是新颖的，并且与草酸钙结晶的已知抑制剂在结构上不相关。因此，它被称为晶体粘附抑制剂或CAI。在包括肾脏，心脏，胰腺，肝脏和睾丸在内的多种大鼠组织中检测到免疫反应性CAI。免疫组织化学显示，CAI存在于肾细胞胞浆中，也存在于质膜上。重要的是，CAI存在于正常人的尿液中，可以使用草酸钙一水合物晶体亲和色谱从中纯化。 CAI可能是抵抗晶体附着于肾小管细胞以及随后体内肾结石发展的重要防御手段。

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