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首页> 外文期刊>American Journal of Physiology >Gastrin regulates the heparin-binding epidermal-like growth factor promoter via a PKC/EGFR-dependent mechanism.
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Gastrin regulates the heparin-binding epidermal-like growth factor promoter via a PKC/EGFR-dependent mechanism.

机译：胃泌素通过PKC / EGFR依赖性机制调节肝素结合表皮样生长因子启动子。

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摘要

Gastrin is a known growth/differentiation factor for the gastric mucosa. Its effects are likely mediated by the induction of heparin-binding epidermal-like growth factor (HB-EGF), a member of the EGF family of growth factors that is expressed by gastric parietal cells. In this study, we investigated the regulation of the HB-EGF promoter by gastrin in a human gastric cancer cell line. Serial human HB-EGF promoter-luciferase reporter deletion constructs and heterologous promoter constructs were transfected into AGS-E cells and stimulated with gastrin (10(-7) M) with or without various signal transduction inhibitors. EMSA were also performed. Gastrin stimulation resulted in a fivefold increase in HB-EGF-luciferase activity. The cis-acting element mediating gastrin responsiveness was mapped to the -69 to -58 region of the HB-EGF promoter. Gastrin stimulation was PKC dependent and at least partially mediated by activation of the EGF receptor.
机译：胃泌素是胃粘膜的已知生长/分化因子。它的作用可能是由肝素结合表皮样生长因子（HB-EGF）的诱导介导的，后者是胃壁细胞表达的EGF生长因子家族的成员。在这项研究中，我们研究了胃泌素在人胃癌细胞系中对HB-EGF启动子的调节。将连续的人HB-EGF启动子-荧光素酶报告基因缺失构建体和异源启动子构建体转染到AGS-E细胞中，并用胃泌素（10（-7）M）刺激或不刺激各种信号转导抑制剂。还进行了EMSA。胃泌素刺激导致HB-EGF荧光素酶活性增加了五倍。介导胃泌素反应性的顺式作用元件被定位到HB-EGF启动子的-69至-58区域。胃泌素刺激是PKC依赖性的，并且至少部分由EGF受体的激活介导。

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