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首页> 外文期刊>American Journal of Physiology >Castration inhibits exercise-induced accumulation of Hsp70 in male rodent hearts.
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Castration inhibits exercise-induced accumulation of Hsp70 in male rodent hearts.

机译：去势抑制雄性啮齿动物心脏运动引起的Hsp70积累。

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摘要

Intense exercise leads to accumulation of the inducible member of the 70-kDa family of heat shock proteins, Hsp70, in male, but not female, hearts. Estrogen is at least partially responsible for this difference. Because androgen receptors are expressed in the heart and castration leads to decreases in calcium regulatory proteins and altered cardiac function, testosterone (T) or its metabolites could also be involved. We hypothesized that removal of endogenous T production through castration would reduce cardiac Hsp70 accumulation after an acute exercise bout, whereas castrated animals supplemented with 5alpha-dihydrotestosterone (DHT) would show the intact male response. Fifty-four 8-wk-old male Sprague-Dawley rats were divided into intact, castrated, or castrated + DHT groups (n = 18/group). At 11 wk of age, 12 animals in each group undertook a 60-min bout of treadmill running at 30 m/min (2% incline) while the remaining 6 in each group remained sedentary. At 30 min or 24 h after exercise (n = 6/time point), blood and hearts were harvested for analysis. Serum T was undetectable in castrated and DHT-treated castrated rats, whereas serum DHT was significantly reduced in castrated animals only (approximately 60% reduction) (P < 0.05). Although there were no differences in constitutive levels of Hsp70 protein, exercise significantly increased cardiac hsp70 mRNA and protein in intact and DHT-supplemented rats, but not in castrated animals (P < 0.05). To examine whether castration eliminated the ability to respond to stress, another six intact and six castrated animals were subjected to a 15-min period of hyperthermia (core temperature raised to 42 degrees C) and killed 24 h later. As opposed to exercise, castrated animals subjected to heat shock exhibited increases in Hsp70 above nonshocked (i.e., sedentary) animals, similarly to intact males (P < 0.05). These data suggest that androgens, in addition to estrogen, play a role in the sexual dimorphism observed in the stress response to exercise but not heat shock.
机译：剧烈运动会导致男性心脏（而非女性心脏）中70 kDa热休克蛋白Hsp70的诱导型成员蓄积。雌激素至少部分负责这种差异。由于雄激素受体在心脏中表达，去势导致钙调节蛋白减少和心脏功能改变，因此睾丸激素（T）或其代谢产物也可能参与其中。我们假设通过cast割去除内源性T产生会减少急性运动发作后心脏Hsp70的积累，而补充5α-二氢睾丸激素（DHT）的cast割动物将表现出完整的雄性反应。将54只8周大的雄性Sprague-Dawley大鼠分为完整，去势或去势+ DHT组（n = 18 /组）。在11周龄时，每组12只动物以30 m / min（倾斜2％）的速度进行了60分钟的跑步机运动，而每组中其余6只仍久坐。运动后30分钟或24小时（n = 6 /时间点），采集血液和心脏进行分析。在去势和DHT治疗的去势大鼠中无法检测到血清T，而仅在去势动物中血清DHT显着降低（降低了约60％）（P <0.05）。尽管Hsp70蛋白质的组成水平没有差异，但运动在完整和补充DHT的大鼠中心脏hsp70 mRNA和蛋白质显着增加，而在cast割的动物中则没有（P <0.05）。为了检查去势是否消除了对压力的反应能力，对另外六只完整的和六只去势的动物进行了15分钟的高温治疗（核心温度升至42摄氏度），并在24小时后处死。与运动相反，受cast割的动物遭受热激后，其Hsp70的升高要高于未受惊吓（即久坐）的动物，与完整雄性动物相似（P <0.05）。这些数据表明，除了雌激素外，雄激素还在运动应激反应中观察到的性二态性中起作用，而不是热休克。

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