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首页> 外文期刊>American Journal of Physiology >Ultrastructural localization of UT-A and UT-B in rat kidneys with different hydration status.
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Ultrastructural localization of UT-A and UT-B in rat kidneys with different hydration status.

机译：UT-A和UT-B在不同水化状态的大鼠肾脏中的超微结构定位。

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摘要

Urea transport in the kidney is mediated by a family of transporter proteins, including renal urea transporters (UT-A) and erythrocyte urea transporters (UT-B). We aimed to determine whether hydration status affects the subcellular distribution of urea transporters. Male Sprague-Dawley rats were divided into three groups: dehydrated rats (WD) given minimum water, hydrated rats (WL) given 3% sucrose in water for 3 days before death, and control rats given free access to water. We labeled kidney sections with antibodies against UT-A1 and UT-A2 (L194), UT-A3 (Q2), and UT-B using preembedding immunoperoxidase and immunogold methods. In control animals, UT-A1 and UT-A3 immunoreactivities were observed throughout the cytoplasm in inner medullary collecting duct (IMCD) cells, and weak labeling was observed on the basolateral plasma membrane. UT-A2 immunoreactivity in the descending thin limbs (DTL) was observed mainly on the apical and basolateral membranes of type I epithelium, and very faint labeling was observed in the long-loop DTL at the border between the outer and inner medulla. UT-A1 immunoreactivity intensity was markedly lower, and UT-A3 immunoreactivity was higher in IMCD of WD vs. controls. UT-A2 immunoreactivity intensities in the plasma membrane and cytoplasm of type I, II, and III epithelia of DTL were greater in WD vs. controls. In contrast, UT-A1 expression was greater and UT-A2 and UT-A3 expressions were lower in WL vs. controls. The subcellular distribution of UT-A in DTL or IMCD did not differ between control and experimental animals. UT-B was expressed in the plasma membrane of the descending vasa recta of both control and experimental animals. UT-B intensity was higher in WD and lower in WL vs. controls. These data indicate that changes in hydration status over 3 days affected urea transporter protein expression without changing its subcellular distribution.
机译：肾脏中的尿素转运是由一系列转运蛋白介导的，包括肾脏尿素转运蛋白（UT-A）和红细胞尿素转运蛋白（UT-B）。我们旨在确定水合状态是否影响尿素转运蛋白的亚细胞分布。将雄性Sprague-Dawley大鼠分为三组：死亡前3天给予最低水的脱水大鼠（WD），给予水中3％蔗糖的含水大鼠（WL）和自由饮水的对照组大鼠。我们使用预先包埋的免疫过氧化物酶和免疫金方法，用针对UT-A1和UT-A2（L194），UT-A3（Q2）和UT-B的抗体标记肾脏切片。在对照动物中，在整个髓内收集管（IMCD）细胞的整个细胞质中均观察到UT-A1和UT-A3免疫反应性，并且在基底外侧质膜上观察到弱标记。下降的四肢（DTL）中的UT-A2免疫反应性主要在I型上皮的顶膜和基底外侧膜上观察到，而在外延髓膜和内延髓膜之间的边界的长环DTL中观察到非常微弱的标记。与对照组相比，WD IMCD中的UT-A1免疫反应强度明显较低，而UT-A3免疫反应强度较高。与对照相比，WD的DTL的I，II和III型上皮细胞膜和细胞质中的UT-A2免疫反应强度更高。相反，与对照相比，WL中的UT-A1表达较高，而UT-A2和UT-A3表达较低。在对照和实验动物之间，DTL或IMCD中UT-A的亚细胞分布没有差异。 UT-B在对照动物和实验动物的降脉络膜的质膜中表达。与对照相比，WD中的UT-B强度较高，而WL中的UT-B强度较低。这些数据表明，超过3天的水合状态变化会影响尿素转运蛋白的表达，而不会改变其亚细胞分布。

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