...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Food-induced expression of orexin receptors in rat duodenal mucosa regulates the bicarbonate secretory response to orexin-A.
【24h】

Food-induced expression of orexin receptors in rat duodenal mucosa regulates the bicarbonate secretory response to orexin-A.

机译：食物诱导的大鼠十二指肠粘膜中食欲素受体的表达调节对食欲素A的碳酸氢盐分泌反应。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Presence of appetite-regulating peptides orexin-A and orexin-B in mucosal endocrine cells suggests a role in physiological control of the intestine. Our aim was to characterize orexin-induced stimulation of duodenal bicarbonate secretion and modulation of secretory responses and mucosal orexin receptors by overnight food deprivation. Lewis x Dark Agouti rats were anesthetized and proximal duodenum cannulated in situ. Mucosal bicarbonate secretion (pH stat) and mean arterial blood pressure were continuously recorded. Orexin-A was administered intra-arterially close to the duodenum, intraluminally, or into the brain ventricles. Total RNA was extracted from mucosal specimens, reverse transcribed to cDNA and expression of orexin receptors 1 and 2 (OX1 and OX2) measured by quantitative real-time PCR. OX1 protein was measured by Western blot. Intra-arterial orexin-A (60-600 nmol.h(-1).kg(-1)) increased (P < 0.01) the duodenal secretion in fed but not in fasted animals. The OX1 receptor antagonist SB-334867, which was also found to have a partial agonist action, abolished the orexin-induced secretory response but did not affect secretion induced by the muscarinic agonist bethanechol. Atropine, in contrast, inhibited bethanechol but not orexin-induced secretion. Orexin-A infused into the brain ventricles (2-20 nmol.kg(-1).h(-1)) or added to luminal perfusate (1.0-100 nM) did not affect secretion, indicating that orexin-A acts peripherally and at basolateral receptors. Overnight fasting decreased mucosal OX1 and OX2 mRNA expression (P < 0.01) as well as OX1 protein expression (P < 0.05). We conclude that stimulation of secretion by orexin-A may involve both receptor types and is independent of cholinergic pathways. Intestinal OX receptors and secretory responses are markedly related to food intake.
机译：粘膜内分泌细胞中食欲调节肽orexin-A和orexin-B的存在提示了肠道的生理控制。我们的目标是通过过夜的食物剥夺来表征食欲素诱导的十二指肠碳酸氢盐分泌的刺激以及分泌反应和粘膜食欲素受体的调节。麻醉Lewis x Dark Agouti大鼠，并在原位插管十二指肠近端。连续记录粘膜碳酸氢盐分泌（pH stat）和平均动脉血压。 Orexin-A在靠近十二指肠的动脉内，腔内或脑室给药。从粘膜标本中提取总RNA，逆转录为cDNA，并通过实时定量PCR测定orexin受体1和2（OX1和OX2）的表达。通过蛋白质印迹法测定OX1蛋白。进食但未禁食动物的动脉内orexin-A（60-600 nmol.h（-1）.kg（-1））增加（P <0.01）十二指肠分泌。还被发现具有部分激动剂作用的OX1受体拮抗剂SB-334867消除了由食欲素诱导的分泌反应，但不影响由毒蕈碱激动剂苯乙二酚诱导的分泌。相反，阿托品抑制安息香但不抑制由食欲素诱导的分泌。将Orexin-A注入脑室（2-20 nmol.kg（-1）.h（-1））或添加至腔内灌注液（1.0-100 nM）不会影响分泌，这表明Orexin-A在周围和周围起作用。在基底外侧受体处。过夜禁食可降低粘膜OX1和OX2 mRNA表达（P <0.01）以及OX1蛋白表达（P <0.05）。我们得出的结论是，orexin-A刺激分泌可能涉及两种受体类型，并且独立于胆碱能途径。肠道OX受体和分泌反应与食物摄入量显着相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号