• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Cadmium and calcium uptake in isolated mitochondria-rich cell populations from the gills of the freshwater rainbow trout.
【24h】

Cadmium and calcium uptake in isolated mitochondria-rich cell populations from the gills of the freshwater rainbow trout.

机译：从淡水虹鳟鱼的isolated中分离出的富含线粒体的细胞群中的镉和钙吸收。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel cell isolation technique was used to characterize cadmium and calcium uptake in distinct populations of gill cells from the adult rainbow trout (Oncorhynchus mykiss). A specific population of mitochondria-rich (MR) cell, termed the PNA+ MR cell (PNA is peanut lectin agglutinin), was found to accumulate over threefold more 109Cd than did PNA- MR cells, pavement cells (PV cells), and mucous cells during a 1-h in vivo exposure at 2.4 microg/l 109Cd. In vitro 109Cd exposures, performed in standard PBS and Cl- -free PBS, at concentrations from 1 to 16 microg/l 109Cd, were also carried out to further characterize Cd2+ uptake kinetics. As observed during in vivo experiments, PNA+ MR cells accumulated significantly more 109Cd than did other cell types when exposures were performed by an in vitro procedure in PBS. Under such conditions, Cd2+ accumulation kinetics in all cell types could be described with Michaelis-Menten relationships, with Km values of approximately 3.0 microg/l Cd (27 nM) for both MR cell subtypes and 8.6 microg/l Cd (77 nM) for PV cells. In similar experiments performed in Cl- -free conditions, a significant reduction in 109Cd accumulation in PNA+ MR cells was seen but not in PNA- MR or in PV cells. In vitro 45Ca fluxes were also performed to determine the cellular localization of Ca2+ transport in these functionally distinct populations of gill cells. 45Ca uptake was most pronounced in PNA+ MR cells, with levels over threefold higher than those found in either PNA(-) MR or in PV cells. Results from the present study suggest that the PNA+ MR cell type is a high-affinity and high-capacity site for apical entry of Cd2+ and Ca2+ in the gill epithelium of rainbow trout.
机译：一种新颖的细胞分离技术被用来表征成年虹鳟（Oncorhynchus mykiss）distinct细胞不同种群中镉和钙的吸收。发现特定的线粒体富集（MR）细胞群体（称为PNA + MR细胞（PNA是花生凝集素凝集素））积累的109Cd比PNA- MR细胞，路面细胞（PV细胞）和黏液细胞高出三倍以上在以2.4 microg / l 109Cd进行1小时体内暴露期间。还进行了标准PBS和无氯PBS中浓度为1至16微克/升109Cd的体外109Cd暴露，以进一步表征Cd2 +吸收动力学。如在体内实验中观察到的，当通过体外程序在PBS中进行暴露时，PNA + MR细胞比其他细胞类型积累的109Cd明显多。在这种条件下，所有细胞类型中Cd2 +的蓄积动力学可以用Michaelis-Menten关系描述，两种MR细胞亚型的Km值均约为3.0 microg / l Cd（27 nM），而对于MR细胞亚型，其Km值约为8.6 microg / l Cd（77 nM）。光伏电池。在无氯条件下进行的类似实验中，观察到PNA + MR细胞中109Cd积累的显着减少，而PNA-MR或PV细胞中则没有。还进行了体外45Ca流量测定，以确定这些功能不同的distinct细胞群体中Ca2 +转运的细胞定位。在PNA + MR细胞中，最明显的是45Ca吸收，其水平比PNA（-）MR或PV细胞中的高出三倍。本研究的结果表明，PNA + MR细胞类型是虹鳟the腮上皮中Cd2 +和Ca2 +顶端进入的高亲和力和高容量位点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号