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首页> 外文期刊>American Journal of Physiology >The importance of limitations in aerobic metabolism, glycolysis, and membrane excitability for the development of high-frequency fatigue in isolated rat soleus muscle.
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The importance of limitations in aerobic metabolism, glycolysis, and membrane excitability for the development of high-frequency fatigue in isolated rat soleus muscle.

机译：有氧代谢，糖酵解和膜兴奋性方面的限制对于离体大鼠比目鱼肌高频疲劳发展的重要性。

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We investigated the role of limitations in aerobic metabolism, glycolysis, and membrane excitability for development of high-frequency fatigue in isolated rat soleus muscle. Muscles mounted on force transducers were incubated in buffer bubbled with 5% CO(2) and either 95% O(2) (oxygenated) or 95% N(2) (anoxic) and stimulated at 60 Hz continuously for 30-120 s or intermittently for 120 s. Cyanide (2 mM) and 2-deoxyglucose (10 mM) were used to inhibit aerobic metabolism and both glycolysis and aerobic metabolism, respectively. Excitability was reduced by carbacholine (10 microM), a nicotinic ACh receptor agonist, or ouabain (10 microM), an Na(+)-K(+) pump inhibitor. Membrane excitability was measured by recording M waves. Intracellular Na(+) and K(+) contents and membrane potentials were measured by flame photometry and microelectrodes, respectively. During 120 s of continuous stimulation, oxygenated and anoxic muscles showed the same force loss. In oxygenated muscles, cyanide did not alter force loss for up to 90 s, whereas 2-deoxyglucose increased force loss (by 19-69%; P < 0.01) from 14 s of stimulation. In oxygenated muscles, 60 s of stimulation reduced force, M wave area, and amplitude by 70-90% (P < 0.001). Carbacholine or ouabain increased intracellular Na(+) content (P < 0.001), induced a 7- to 8-mV membrane depolarization (P < 0.001), and accelerated the rate of force loss (by 250-414%) during 30 s of stimulation (P < 0.001). Similar effects were seen with intermittent stimulation. In conclusion, limitations in glycolysis and subsequently also in aerobic metabolism, as well as membrane excitability but not aerobic metabolism alone, appear to play an important role in the development of high-frequency fatigue in isolated rat soleus muscle.
机译：我们调查了有氧代谢，糖酵解和膜兴奋性方面的局限性，在离体大鼠比目鱼肌中产生高频疲劳。将安装在力传感器上的肌肉在装有5％CO（2）和95％O（2）（含氧）或95％N（2）（缺氧）的缓冲液中孵育，并在60 Hz下连续刺激30-120 s或间歇地持续120 s。氰化物（2 mM）和2-脱氧葡萄糖（10 mM）分别用于抑制有氧代谢，糖酵解和有氧代谢。烟碱ACh受体激动剂carbacholine（10 microM）或Na（+）-K（+）泵抑制剂ouabain（10 microM）降低了兴奋性。通过记录M波来测量膜的兴奋性。分别通过火焰光度法和微电极测量细胞内Na（+）和K（+）含量以及膜电位。在持续刺激的120 s内，充氧和缺氧的肌肉显示出相同的力损失。在含氧的肌肉中，氰化物在长达90 s的时间内不会改变力的损失，而2-脱氧葡萄糖在14 s的刺激后会增加力的损失（19-69％; P <0.01）。在含氧的肌肉中，60 s的刺激可使力，M波面积和振幅降低70-90％（P <0.001）。 Carbacholine或哇巴因增加细胞内Na（+）含量（P <0.001），诱导7到8 mV的膜去极化（P <0.001），并在30 s的时间内加速力损失率（250-414％）。刺激（P <0.001）。间歇性刺激也有类似的效果。总之，糖酵解的限制以及随后的有氧代谢的限制，以及膜兴奋性的限制，而不仅仅是有氧代谢的限制，似乎在离体大鼠比目鱼肌的高频疲劳发展中起着重要作用。

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