• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >AQP7 is localized in capillaries of adipose tissue, cardiac and striated muscle: implications in glycerol metabolism.
【24h】

AQP7 is localized in capillaries of adipose tissue, cardiac and striated muscle: implications in glycerol metabolism.

机译：AQP7位于脂肪组织，心肌和横纹肌的毛细血管中：对甘油代谢的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aquaporin (AQP7) is expressed in proximal tubules and is involved in glycerol uptake. The cellular expression and physiological function in other organs remain largely undefined. AQP7 knockout (KO) mice were generated and used for immunohistochemical analyses to define the organ and cellular expression of AQP7. AQP7 labeling was found in kidney proximal tubule, heart, skeletal muscle, testis, epididymis, as well as in white and brown adipose tissue (WAT and BAT) of wild-type mice. Importantly, immunoreactivity was completely absent from these tissues in AQP7 KO mice. At the cellular level, the capillary endothelium WAT and BAT displayed prominent staining, whereas AQP7 labeling in adipocyte membranes was undetectable. Double-labeling confocal microscopy revealed coexpression of AQP7 with capillary AQP1 but not with adipocyte GLUT4. Moreover, immunoelectron microscopy and RT-PCR of isolated microvessels confirmed the vascular AQP7 expression. Distinct immunolabeling of the capillary endothelium was alsoobserved in both skeletal and heart muscle with no apparent staining of skeletal or cardiac myocytes. As previously reported, specific immunolabeling was confined to brush border in segment 3 renal proximal tubules and to spermatids and spermatozoa in male reproductive tract. The expression of AQP7 was induced up to 2.2-fold in WAT of mice with streptozotocin-induced diabetes mellitus (S-DM) compared with controls and fasting for 72 h (but not 24 h) induced significant increase in AQP7 expression. In conclusion, AQP7 is expressed in capillary endothelia of adipose tissue (and cardiac and striated muscle) and is upregulated in WAT in response to S-DM supporting its role in glycerol metabolism.
机译：水通道蛋白（AQP7）在近端小管中表达，并参与甘油的摄取。其他器官中的细胞表达和生理功能仍未明确。产生AQP7基因敲除（KO）小鼠，用于免疫组织化学分析，以定义AQP7的器官和细胞表达。在野生型小鼠的肾脏近端小管，心脏，骨骼肌，睾丸，附睾以及白色和棕色脂肪组织（WAT和BAT）中发现了AQP7标记。重要的是，AQP7 KO小鼠的这些组织中完全没有免疫反应性。在细胞水平上，毛细血管内皮细胞WAT和BAT表现出明显的染色，而在脂肪细胞膜中的AQP7标记则无法检测到。双标记共聚焦显微镜检查显示，AQP7与毛细管AQP1共表达，但与脂肪细胞GLUT4不共表达。此外，免疫电子显微镜和RT-PCR的分离的微血管证实了血管AQP7的表达。在骨骼肌和心肌中也观察到了毛细血管内皮的不同免疫标记，而骨骼肌或心肌细胞没有明显的染色。如先前报道，特异性免疫标记仅限于第3段肾近端小管的刷状边界以及雄性生殖道中的精子和精子。与对照相比，链脲佐菌素诱导的糖尿病（S-DM）小鼠的WAT中诱导的AQP7的表达高达2.2倍，而禁食72 h（但不是24 h）则导致AQP7的表达显着增加。总之，AQP7在脂肪组织（以及心肌和横纹肌）的毛细血管内皮中表达，并在WAT中上调，以响应S-DM支持其在甘油代谢中的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号