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首页> 外文期刊>American Journal of Physiology >Targeted ablation of mesenteric projecting sympathetic neurons reduces the hemodynamic response to pain in conscious, spinal cord-transected rats
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Targeted ablation of mesenteric projecting sympathetic neurons reduces the hemodynamic response to pain in conscious, spinal cord-transected rats

机译：肠系膜投射交感神经元的靶向消融可降低自觉脊髓横断大鼠对疼痛的血液动力学反应

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Individuals with spinal cord injuries above thoracic level 6 (T6) experience episodic bouts of life-threatening hypertension as part of a condition termed autonomic dysreflexia. The paroxysmal hypertension can be caused by a painful stimulus below the level of the injury. Targeted ablation of mesenteric projecting sympathetic neurons may reduce the severity of autonomic dysreflexia by reducing sympathetic activity. Therefore, cholera toxin B subunit (CTB) conjugated to saporin (SAP; a ribosomal inactivating protein that binds to and inactivates ribosomes) was injected into the celiac ganglion to test the hypothesis that targeted ablation of mesenteric projecting sympathetic neurons reduces the pressor response to pain in conscious, spinal cord-transected rats. Nine Sprague-Dawley male rats underwent a spinal cord transection between thoracic vertebrae 4 and 5. Following recovery (5 wk), all rats were instrumented with a radio telemetry device for recording arterial pressure and bilateral catheters in the gluteus maximus muscles for the infusion of hypertonic saline (hNa+Cl-). Subsequently, the hemodynamic responses to intramuscular injection of hNa+Cl- (100 μl and 250 μl, in random order) were determined. Following the experiments in the no celiac ganglia injected condition (NGI), rats received injections of CTB-SAP (n = 5) or CTB (n = 3) into the celiac ganglia. CTB-SAP rats, compared with NGI and CTB rats, had reduced pressor responses to hNa+Cl -. Furthermore, the number of stained neurons in the celiac ganglia and spinal cord (segments T6-T12), was reduced in CTB-SAP rats. Thus, CTB-SAP retrogradely transported from the celiac ganglia is effective at ablating mesenteric projecting sympathetic neurons and reducing the pressor response to pain in spinal cord-transected rats.
机译：脊髓损伤高于胸部6级（T6）的个体会经历发作性发作的危及生命的高血压，这是称为自主神经反射异常的一部分。阵发性高血压可以由低于损伤水平的疼痛刺激引起。肠系膜投射交感神经元的靶向消融可通过减少交感神经活动来降低自主神经反射不良的严重程度。因此，将与皂草素（SAP;结合并灭活核糖体的核糖体失活蛋白）缀合的霍乱毒素B亚基（CTB）注射到腹腔神经节中，以检验以下假设：靶向消融肠系膜投射交感神经元会降低对疼痛的升压反应在有意识的脊髓横断大鼠中。九只Sprague-Dawley雄性大鼠在胸椎4和5之间进行了脊髓横断。恢复（5周）后，所有大鼠均配备了无线电遥测设备以记录动脉压力和臀大肌中的双侧导管以输注高渗盐水（hNa + Cl-）。随后，确定对肌肉内注射hNa + Cl-（100μl和250μl，以随机顺序）的血液动力学响应。在无腹腔神经节注射条件（NGI）下进行实验后，大鼠接受了将CTB-SAP（n = 5）或CTB（n = 3）注射到腹腔神经节中。与NGI和CTB大鼠相比，CTB-SAP大鼠对hNa + Cl-的升压反应降低。此外，在CTB-SAP大鼠中，腹腔神经节和脊髓（节段T6-T12）中染色的神经元数量减少。因此，从腹腔神经节逆行运输的CTB-SAP在消融肠系膜投射交感神经元和减少脊髓横断大鼠疼痛中的升压反应方面有效。

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