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首页> 外文期刊>American Journal of Physiology >Rhythmic changes in colonic motility are regulated by period genes.
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Rhythmic changes in colonic motility are regulated by period genes.

机译：结肠运动的节奏变化受周期基因调节。

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摘要

Human bowel movements usually occur during the day and seldom during the night, suggesting a role for a biological clock in the regulation of colonic motility. Research has unveiled molecular and physiological mechanisms for biological clock function in the brain; less is known about peripheral rhythmicity. This study aimed to determine whether clock genes such as period 1 (per1) and period2 (per2) modulate rhythmic changes in colonic motility. Organ bath studies, intracolonic pressure measurements, and stool studies were used to examine measures of colonic motility in wild-type and per1per2 double-knockout mice. To further examine the mechanism underlying rhythmic changes in circular muscle contractility, additional studies were completed in neuronal nitric oxide synthase (nNOS) knockout mice. Intracolonic pressure changes and stool output in vivo, and colonic circular muscle contractility ex vivo, are rhythmic with greatest activity at the start of night in nocturnal wild-type mice. In contrast, rhythmicity in these measures was absent in per1per2 double-knockout mice. Rhythmicity was also abolished in colonic circular muscle contractility of wild-type mice in the presence of N(omega)-nitro-L-arginine methyl ester and in nNOS knockout mice. These findings suggest that rhythms in colonic motility are regulated by both clock genes and a nNOS-mediated inhibitory process and suggest a connection between these two mechanisms.
机译：人的排便通常发生在白天，很少发生在夜间，这暗示了生物钟在结肠运动调节中的作用。研究揭示了大脑中生物钟功能的分子和生理机制。关于周围节律性知之甚少。这项研究旨在确定时钟基因（如时期1（per1）和时期2（per2））是否能调节结肠运动的节律变化。器官浸浴研究，结肠内压测量和粪便研究用于检查野生型和per1per2双敲除小鼠中结肠运动的测量。为进一步检查环状肌肉收缩节律性变化的机制，在神经元一氧化氮合酶（nNOS）敲除小鼠中完成了其他研究。在夜间野生型小鼠中，结肠内压变化和体内大便排出量以及离体结肠环形肌肉收缩是节律性最强的节律性。相比之下，per1per2双敲除小鼠中没有这些措施的节律性。在N（ω）-硝基-L-精氨酸甲酯存在下和在nNOS基因敲除小鼠中，野生型小鼠的结肠环形肌肉收缩力的节律性也被消除。这些发现表明结肠运动的节律受时钟基因和nNOS介导的抑制过程的调节，并暗示这两种机制之间存在联系。

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