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首页> 外文期刊>American Journal of Physiology >DIDS inhibits Na-K-ATPase activity in porcine nonpigmented ciliary epithelial cells by a Src family kinase-dependent mechanism
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DIDS inhibits Na-K-ATPase activity in porcine nonpigmented ciliary epithelial cells by a Src family kinase-dependent mechanism

机译：DIDS通过Src家族激酶依赖性机制抑制猪非色素性睫状上皮细胞中的Na-K-ATPase活性

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摘要

The anion transport inhibitor DIDS is known to reduce aqueous humor secretion but questions remain about anion dependence of the effect. In some tissues, DIDS is reported to cause Na-K-ATPase inhibition. Here, we report on the ability of DIDS to inhibit Na-K-ATPase activity in nonpigmented ciliary epithelium (NPE) and investigate the underlying mechanism. Porcine NPE cells were cultured to confluence on permeable supports, treated with drugs added to both sides of the membrane, and then used for ~86Rb uptake measurements or homogenized to measure Na-K-ATPase activity or to detect protein phos-phorylation. DIDS inhibited ouabain-sensitive ~86Rb uptake, activated Src family kinase (SFK), and caused a reduction of Na-K-ATPase activity. PP2, an SFK inhibitor, prevented the DIDS responses. In BCECF-loaded NPE, DIDS was found to reduce cytoplasmic pH (pH_i). PP2-sensitive Na-K-ATPase activity inhibition, ~86Rb uptake suppression, and SFK activation were observed when a similar reduction of pH_i was imposed by low-pH medium or an ammonium chloride withdrawal maneuver. PP2 and the ERK inhibitor U0126 prevented robust ERK1/2 activation in cells exposed to DIDS or subjected to pH_i reduction, but U0126 did not prevent SFK activation or the Na-K-ATPase activity response. The evidence points to an inhibitory influence of DIDS on NPE Na-K-ATPase activity by a mechanism that hinges on SFK activation associated with a reduction of cytoplasmic pH.
机译：已知阴离子转运抑制剂DIDS可减少房水分泌，但是关于阴离子对作用的依赖性仍存在疑问。在某些组织中，据报道DIDS会引起Na-K-ATPase抑制。在这里，我们报告DIDS抑制非色素性睫状上皮细胞（NPE）中的Na-K-ATPase活性的能力，并研究其潜在机制。培养猪NPE细胞使其在可渗透的支持物上汇合，用添加到膜两侧的药物处理，然后用于〜86Rb吸收测量或均质化以测量Na-K-ATPase活性或检测蛋白质的磷酸化。 DIDS抑制了哇巴因敏感的〜86Rb摄取，激活了Src家族激酶（SFK），并导致Na-K-ATPase活性降低。 SFK抑制剂PP2阻止了DIDS反应。在载有BCECF的NPE中，发现DIDS可降低细胞质的pH（pH_i）。当通过低pH介质或氯化铵撤除操作使pH_i降低相似时，可观察到PP2敏感的Na-K-ATPase活性抑制，〜86Rb吸收抑制和SFK活化。 PP2和ERK抑制剂U0126阻止了暴露于DIDS或遭受pH_i降低的细胞中强大的ERK1 / 2活化，但U0126并未阻止SFK活化或Na-K-ATPase活性反应。证据表明，DIDS对NPE Na-K-ATPase活性的抑制作用取决于与细胞质pH降低相关的SFK激活的机制。

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