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首页> 外文期刊>Analytical chemistry >Carbon Nanotubes as a Low Background Signal Platform for a Molecular Aptamer Beacon on the Basis of Long-Range Resonance Energy Transfer
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Carbon Nanotubes as a Low Background Signal Platform for a Molecular Aptamer Beacon on the Basis of Long-Range Resonance Energy Transfer

机译：碳纳米管作为基于远程共振能量转移的分子适体信标的低背景信号平台

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摘要

Although holding the advantages of both an aptamer and a molecular beacon (MB), a molecular aptamer beacon (MAB) needs complicated and expensive modifications at both of its ends and usually has a high background signal because of the low energy transfer efficiency between the donor and the acceptor. To overcome these shortcomings, in this study, we develop a long-range resonance energy transfer (LrRET) system by separating the donor from the acceptor, wherein only one end of the MAB is fluorescently labeled and acts as the energy donor and multiwalled carbon nanotubes (MWCNTs) are introduced as the energy acceptor. To test the feasibility of the newly designed MAB system, adenosine triphosphate (ATP) has been employed as a proof-of-concept target. It is found that the fluorescence of the designed MAB is completely quenched by MWCNTs, supplying a very low background signal. Then the quenched fluorescence is recovered significantly with the addition of ATP, so that ATP can be detected in the range of 0.8-80 (mu)M with a limit of detection of 0.5 (mu)M (3sigma). Compared with the conventional fluorescence resonance energy transfer, the efficiency of LrRET between the dye and MWCNTs is much higher. Since only one end of the MAB needs the modification, the present strategy is simple and cost-effective. Furthermore, the use of MWCNTs can greatly reduce the fluorescence background of the MAB and supply a high sensitivity, showing its generality for detection of a variety of targets.
机译：尽管同时拥有适体和分子信标（MB）的优点，但分子适体信标（MAB）的两端都需要复杂且昂贵的修饰，并且由于供体之间的能量转移效率低，通常具有较高的背景信号和接受者。为了克服这些缺点，在这项研究中，我们通过将供体与受体分离，开发了一种长距离共振能量转移（LrRET）系统，其中仅MAB的一端被荧光标记，并充当能量供体和多壁碳纳米管（MWCNT）被引入作为能量受体。为了测试新设计的MAB系统的可行性，三磷酸腺苷（ATP）已被用作概念验证的目标。发现设计的MAB的荧光被MWCNT完全淬灭，提供了非常低的背景信号。然后，通过添加ATP可以显着地恢复猝灭的荧光，从而可以在0.8-80μM的范围内检测到ATP，检测限为0.5μM（3sigma）。与常规的荧光共振能量转移相比，染料和MWCNT之间的LrRET效率更高。由于仅MAB的一端需要修改，因此本策略简单且具有成本效益。此外，使用MWCNT可以大大降低MAB的荧光背景并提供高灵敏度，显示出其在检测各种靶标中的通用性。

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