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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Bioluminescence Imaging of Adoptively Transferred Lymphocytes During Allogeneic Tumor Rejection
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Bioluminescence Imaging of Adoptively Transferred Lymphocytes During Allogeneic Tumor Rejection

机译：同种异体肿瘤排斥过程中过继转移的淋巴细胞的生物发光成像。

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摘要

Aim: The aim of the present study was to analyze the survival, spatial distribution and proliferation of adoptively transferred lymphocytes in allogeneic tumor rejection. Materials and Methods: Transgenic beta-actin-luc mice that express luciferase were sensitized against SL2 tumors and were used as lymphocyte donors to study the anti-tumor effect in SL2 tumor-bearing lymphocyte-deficient RAG(-/-) mice. Whole-body bioluminescence images of recipient mice were obtained to track the adoptively transferred lymphocytes. Proliferation of lymphocytes was estimated by quantification of photon emission. Results: T lymphocytes sensitized against allogeneic SL2 tumors cured the majority of SL2 tumor-bearing RAG(-/-) mice. Bioluminescence imaging showed that transferred T lymphocytes survived in the spleen and lymph nodes. Tumor rejection was associated with lymphocyte proliferation and migration to the tumor site. Conclusion: Sensitized T lymphocytes from transgenic beta-actin-luc mice reject allogeneic SL2 tumors in RAG(-/-) mice and can be tracked in vivo using bioluminescence imaging.
机译：目的：本研究的目的是分析同种异体肿瘤排斥反应中过继转移淋巴细胞的存活，空间分布和增殖。材料和方法：使表达荧光素酶的转基因β-肌动蛋白-luc小鼠对SL2肿瘤敏感，并用作淋巴细胞供体，以研究SL2荷瘤淋巴细胞缺陷RAG（-/-）小鼠的抗肿瘤作用。获得受体小鼠的全身生物发光图像以追踪过继转移的淋巴细胞。通过光子发射的定量估计淋巴细胞的增殖。结果：对同种异体SL2肿瘤敏感的T淋巴细胞治愈了大多数SL2荷瘤RAG（-/-）小鼠。生物发光成像显示，转移的T淋巴细胞在脾和淋巴结中存活。肿瘤排斥与淋巴细胞增殖和迁移到肿瘤部位有关。结论：来自转基因β-肌动蛋白-luc小鼠的敏化T淋巴细胞排斥RAG（-/-）小鼠中的同种异体SL2肿瘤，并且可以使用生物发光成像在体内进行追踪。

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