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Conversion of the Nikon C1 confocal laser-scanning head for multiphoton excitation on an upright microscope

机译:尼康C1共焦激光扫描头的转换,用于在直立显微镜上进行多光子激发

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摘要

Most commercial laser-scanning imaging systems used for confocal fluorescence microscopy can be readily adapted for use with two-photon fluorescence excitation. We report here on the details of the conversion of the Nikon C1 (product released November 2001) with two channels of nondescanned detection of two-photon-excited fluorescence. One of the goals of the design was to utilize off-the-shelf components as much as possible to minimize the use of custom machining and electronics assembly. We also give some initial characterization of the imaging properties of the system.
机译:用于共焦荧光显微镜的大多数商业激光扫描成像系统都可以很容易地与双光子荧光激发一起使用。我们在这里报告尼康C1(产品于2001年11月发布)的转换细节,该转换具有两个未经扫描的双光子激发荧光检测通道。设计的目标之一是尽可能利用现成的组件,以最大程度地减少定制加工和电子装配的使用。我们还给出了系统成像特性的一些初始特征。

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