首页> 外文期刊>Applied optics >Surface-plasmon microscopy with a two-piece solid immersion lens: bright and dark fields
【24h】

Surface-plasmon microscopy with a two-piece solid immersion lens: bright and dark fields

机译:具有两件式固体浸没透镜的表面等离子体显微镜:明场和暗场

获取原文
获取原文并翻译 | 示例
           

摘要

We report bright-field and dark-field surface-plasmon imaging using a modified solid immersion lens and a commercial objective of moderate NA in the epi configuration. The contrast and resolution are extremely good, giving well-resolved images of protein monolayers both in air and in water. We also describe a two-part solid immersion lens that allows the sample to be moved without degrading the image quality in any observable way. The merits of the two-part lens are discussed and compared to commercially available microscope objectives. Finally, we introduce a simple Green's function model that illustrates the key features of both bright-field and dark-field surface-plasmon imaging.
机译:我们报告使用改良的固体浸没透镜和在Epi配置中适度NA的商业目标的明场和暗场表面等离子体成像。对比度和分辨率都非常好,可以在空气和水中提供清晰分辨的蛋白质单层图像。我们还介绍了一种由两部分组成的固体浸没透镜,它可以移动样品,而不会以任何可观察的方式降低图像质量。讨论了两部分式透镜的优点,并将其与市售的显微镜物镜进行了比较。最后,我们介绍一个简单的格林函数模型,该模型说明了明场和暗场表面等离子体激元成像的关键特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号