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New method for determining the depth of field of microscope systems

机译：确定显微镜系统景深的新方法

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摘要

This paper presents new formulas to determine the depth of field (DOF) of optical and digital microscope systems. Unlike the conventional DOF formula, the new methods consider the interplay of geometric and diffraction optics for infinite and finite optical microscopes and for corresponding digital microscope systems. It is shown that in addition to the well understood parameters such as numerical apertures, focal length, and light wavelength, system components such as aperture stops also affect the DOF. For the same objective lens, the DOF is inversely proportional to the size of the aperture stop, and it is proportional to the focal length of the ocular lens. It is also shown that under optimal viewing and operating conditions, the visual accommodation of human observers has no meaningful impact on DOF. The new formulas reported are useful for accurately calculating the DOF of microscopes.
机译：本文提出了确定光学和数字显微镜系统的景深（DOF）的新公式。与传统的自由度公式不同，新方法考虑了无限和有限光学显微镜以及相应的数字显微镜系统的几何光学和衍射光学的相互作用。结果表明，除了众所周知的参数（如数值孔径，焦距和光波长）外，系统组件（如孔径光阑）也会影响自由度。对于同一物镜，DOF与孔径光阑的大小成反比，并且与目镜的焦距成正比。还表明，在最佳观看和操作条件下，人类观察者的视觉适应度对自由度没有任何有意义的影响。报告的新公式可用于准确计算显微镜的自由度。

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