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A facile method for high-performance multicolor upconversion microrods for biological encoding

机译：一种用于生物编码的高性能多色上转换微棒的简便方法

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摘要

In this paper, we demonstrate a facile method for preparing high-performance multicolor upconversion (UC) microrods for biological encoding. Multicolor UC microrods were prepared through a one-step facile hydrothermal method. The as-prepared UC microrods were uniform in shape and size (about 2 mu m in length). For bioconjugation, the UC microrods were functionalized by coating with an amino-terminated silica shell. In order to magnify the bioactive sites, poly (acrylic acid) was introduced to the surface of UC microrods. The optical micrographs displayed that the carboxylated UC microrods were bright enough for observation of single crystals by a conventional microscope. They also exhibited excellent fluorescence stability against time expansion and pH change. Furthermore, a conventional optical microscope can readout the results of a sandwich immunoassay that was conducted by the UC microrods. All the results indicated that the UC microrods exhibited great potential to be new encoding particles for biological molecules.
机译：在本文中，我们演示了一种用于生物编码的高性能多色上转换（UC）微棒制备方法。多色UC微棒是通过一步式简便水热法制备的。所制备的UC微棒的形状和大小均一（长度约为2微米）。对于生物缀合，通过用氨基末端的二氧化硅壳包被，使UC微棒功能化。为了扩大生物活性位点，将聚丙烯酸引入到UC微棒的表面。光学显微照片显示，羧化的UC微棒足够明亮，可以用常规显微镜观察单晶。它们还表现出出色的荧光稳定性，可抵抗时间膨胀和pH值变化。此外，常规的光学显微镜可以读出由UC微棒进行的夹心免疫分析的结果。所有结果表明，UC微棒具有成为生物分子的新编码颗粒的巨大潜力。

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