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Enhanced proliferation of PC12 neural cells on untreated, nanotextured glass coverslips

机译：未经处理的纳米纹理玻璃盖玻片可增强PC12神经细胞的增殖

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Traumatic injury to the central nervous system is a significant health problem. There is no effective treatment available partly because of the complexity of the system. Implementation of multifunctional micro-and nano-device based combinatorial therapeutics can provide biocompatible and tunable approaches to perform on-demand release of specific drugs. This can help the damaged cells to improve neuronal survival, regeneration of axons, and their reconnection to appropriate targets. Nano-topological features induced rapid cell growth is especially important towards the design of effective platforms to facilitate damaged neural circuit reconstruction. In this study, for the first time, feasibility of neuron-like PC12 cell growth on untreated and easy to prepare nanotextured surfaces has been carried out. The PC12 neuron-like cells were cultured on micro reactive ion etched. nanotextured glass coverslips. The effect of nanotextured topology as physical cue for the growth of PC12 cells was observed exclusively, eliminating the possible influence(s) of the enhanced concentration of coated materials on the surface. The cell density was observed to increase by almost 200% on nanotextured coverslips compared to plain coverslips. The morphology study indicated that PC12 cell attachment and growth on the nanotextured substrates did not launch any apoptotic machinery of the cell. Less than 5% cells deformed and depicted condensed nuclei with apoptotic bodies on nanotextured surfaces which is typical for the normal cell handling and culture. Enhanced PC12 cell proliferation by such novel and easy to prepare substrates is not only attractive for neurite outgrowth and guidance, but may be used to increase the affinity of similar cancerous cells (ex: B35 neuroblastoma) and rapid proliferation thereafter-towards the development of combinatorial theranostics to diagnose and treat aggressive cancers like neuroblastoma.
机译：中枢神经系统的外伤是一个重大的健康问题。部分由于系统的复杂性，没有有效的治疗方法。基于多功能微型和纳米设备的组合疗法的实施可以提供生物相容性和可调性方法，以按需释放特定药物。这可以帮助受损细胞改善神经元存活，轴突再生以及将其重新连接至适当的靶标。诱导细胞快速生长的纳米拓扑特征对于设计有效平台以促进受损的神经回路重建尤其重要。在这项研究中，首次进行了神经元样PC12细胞在未经处理且易于制备的纳米纹理表面上生长的可行性。 PC12神经元样细胞在微反应离子蚀刻后培养。纳米纹理的玻璃盖玻片。仅观察到了纳米纹理拓扑结构作为PC12细胞生长的物理线索的作用，消除了表面涂层材料浓度增加的可能影响。与普通盖玻片相比，纳米纹理盖玻片的细胞密度增加了近200％。形态学研究表明，PC12细胞在纳米纹理底物上的附着和生长不会启动细胞的任何凋亡机制。不到5％的细胞变形，并在纳米纹理化的表面上描绘出具有凋亡小体的浓缩核，这对于正常的细胞处理和培养是典型的。通过这种新颖且易于制备的底物增强的PC12细胞增殖不仅对神经突向外生长和引导具有吸引力，而且可用于增加类似癌细胞（例如B35神经母细胞瘤）的亲和力，并随后快速增殖，从而促进组合疗法的发展。诊断和治疗侵袭性癌症（例如神经母细胞瘤）的治疗学。

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