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首页> 外文期刊>Nucleic Acids Research >Major alteration in coxsackievirus B3 genomic RNA structure distinguishes a virulent strain from an avirulent strain
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Major alteration in coxsackievirus B3 genomic RNA structure distinguishes a virulent strain from an avirulent strain

机译：柯萨奇病毒B3基因组RNA结构的重大变化将有毒菌株与无毒菌株区分开来

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摘要

Coxsackievirus B3 (CV-B3) is a cardiovirulent enterovirus that utilizes a 5' untranslated region (5' UTR) to complete critical viral processes. Here, we directly compared the structure of a 5' UTR from a virulent strain with that of a naturally occurring avirulent strain. Using chemical probing analysis, we identified a structural difference between the two 5' UTRs in the highly substituted stem-loop II region (SLII). For the remainder of the 5' UTR, we observed conserved structure. Comparative sequence analysis of 170 closely related enteroviruses revealed that the SLII region lacks conservation. To investigate independent folding and function, two chimeric CV-B3 strains were created by exchanging nucleotides 104-184 and repeating the 5' UTR structural analysis. Neither the parent SLII nor the remaining domains of the background 5' UTR were structurally altered by the exchange, supporting an independent mechanism of folding and function. We show that the attenuated 5' UTR lacks structure in the SLII cardiovirulence determinant.
机译：柯萨奇病毒B3（CV-B3）是一种心脏毒性肠病毒，它利用5'非翻译区（5'UTR）完成关键的病毒过程。在这里，我们直接比较了来自毒株的5'UTR和天然存在的无毒株的结构。使用化学探测分析，我们确定了高度取代的茎环II区（SLII）中两个5'UTR之间的结构差异。对于5'UTR的其余部分，我们观察到了保守的结构。对170种紧密相关的肠病毒的比较序列分析表明，SLII区缺乏保守性。为了研究独立的折叠和功能，通过交换核苷酸104-184并重复5'UTR结构分析来产生两个嵌合的CV-B3菌株。交换不改变亲本SLII或背景5'UTR的其余结构域，从而支持独立的折叠和功能机制。我们显示，减毒的5'UTR在SLII心脏毒性决定簇中缺乏结构。

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