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首页> 外文期刊>Optics Letters >Immune cell imaging using multi-spectral optoacoustic tomography
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Immune cell imaging using multi-spectral optoacoustic tomography

机译:使用多光谱光声层析成像的免疫细胞成像

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摘要

Multispectral optoacoustic tomography (MSOT) offers the potential to image in high-resolution cells tagged with optical labels. In contrast to single wavelength imaging, multispectral excitation and spectral unmixing can differentiate labeled moieties over tissue absorption in the absence of background measurements. This feature can enable longitudinal cellular biology studies well beyond the depths reached by optical microscopy. However, the relation between spectrally resolved fluorescently labeled cells and optoacoustic detection has not been systematically investigated. Herein, we measured titrations of fluorescently labeled cells and establish the optoacoustic signal generated by these cells as a function of cell number and across different cell types. We then assess the MSOT sensitivity to resolve cells implanted in animals.
机译:多光谱光声层析成像(MSOT)提供了在带有光学标记的高分辨率细胞中成像的潜力。与单波长成像相反,在没有背景测量的情况下,多光谱激发和光谱解混可以在组织吸收方面区分标记的部分。此功能可以使纵向细胞生物学研究远远超出光学显微镜所能达到的深度。但是,尚未系统地研究光谱分辨的荧光标记细胞与光声检测之间的关系。在这里,我们测量了荧光标记细胞的滴定度,并建立了由这些细胞产生的光声信号,作为细胞数量和不同细胞类型的函数。然后,我们评估MSOT敏感性来解析植入动物的细胞。

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