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首页> 外文期刊>Optics Letters >Self-calibrating lensless inline-holographic microscopy by a sample holder with reference structures
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Self-calibrating lensless inline-holographic microscopy by a sample holder with reference structures

机译：通过带有参考结构的样品架进行自动校准的无透镜在线全息显微镜

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摘要

A self-calibration technique for lensless compact chip-microscopes based on inline holography with pinhole illumination is presented. The pinhole illumination wave acts as reference and is needed for the reconstruction process. This reference wave is assumed to be spherical, so that its phase is already determined by the position of the pinhole in relation to the image sensor. It is shown that the reconstructed spatial resolution is strongly dependent on the estimation for the pinhole to sensor distance. A precision in the range of tens of microns was reached for microscopic imaging with a spatial resolution in the range of one micron. Therefore additional reference crosses are prepared lithographically on the sample holder. The hologram, which contains the optical information about the sample as well as the reference crosses, is used for calibration and image reconstruction at the same time. The presented technique was tested to allow the reconstruction of a spatial resolution corresponding to the limit of detection apertures of about 0.66. The technique was applied to holograms of test beads and blood smear samples.
机译：提出了一种基于带针孔照明的在线全息技术的无透镜紧凑型芯片显微镜自校准技术。针孔照明波充当参考，是重建过程所需要的。假定该参考波为球形，因此其相位已经由针孔相对于图像传感器的位置确定。结果表明，重建的空间分辨率在很大程度上取决于对针孔到传感器距离的估计。对于显微成像，空间分辨率在1微米的范围内，精度达到了几十微米。因此，在样品架上光刻制备了其他参考十字。包含有关样品的光学信息以及参考十字的全息图可同时用于校准和图像重建。测试提出的技术以允许重建对应于约0.66的检测孔径极限的空间分辨率。该技术已应用于测试珠和血液涂片样本的全息图。

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