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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Novel pyrazoline-based fluorescent probe for detecting thiols and its application in cells
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Novel pyrazoline-based fluorescent probe for detecting thiols and its application in cells

机译：基于吡唑啉的新型硫醇荧光探针及其在细胞中的应用

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摘要

A new compound, N-(4-(1,5-diphenyl-4,5-dihydro-1H-pyrazol-3-yl)phenyl)-acrylamide (probe L), was designed and synthesized as a highly sensitive and selective fluorescent probe for recognizing and detecting thiol from other amino acids. On being mixed with thiol in buffered DMSO:HEPES = 1:1 solution at pH 7.4, the probe exhibited the blue emission at 474 nm. This probe is very sensitive and displayed a linear fluorescence off-on response to thiol. The fluorescence emission of the probe is pH independent in the physiological pH range. Living cell imaging of HeLa cells confirmed its cell permeability and its ability to selectively detect thiol in cells. The structure of the probe was characterized by IR, NMR and HRMS spectroscopy analysis. (C) 2014 Elsevier B.V. All rights reserved.
机译：设计并合成了一种新的化合物N-（4-（1,5-二苯基-4,5-二氢-1H-吡唑-3-基）苯基）-丙烯酰胺（探针L），作为高灵敏度和选择性的荧光剂识别和检测其他氨基酸硫醇的探针。在pH 7.4的DMSO：HEPES = 1：1缓冲溶液中与硫醇混合后，探针在474 nm处显示蓝色发射。该探针非常敏感，并且显示出对硫醇的线性荧光关闭响应。在生理pH范围内，探针的荧光发射与pH无关。 HeLa细胞的活细胞成像证实了其细胞渗透性以及选择性检测细胞中硫醇的能力。探针的结构通过IR，NMR和HRMS光谱分析来表征。 （C）2014 Elsevier B.V.保留所有权利。

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