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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Studies on the binding of fulvic acid with transferrin by spectroscopic analysis
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Studies on the binding of fulvic acid with transferrin by spectroscopic analysis

机译：光谱分析研究富里酸与转铁蛋白的结合

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Transferrin has shown potential in the delivery of anticancer drugs into primarily proliferating cancer cells that over-express transferrin receptors. Fulvic acid has a wide range of biological and pharmacological activities which caused widespread concerns, the interaction of fulvic acid with human serum transferrin (Tf) has great significance for gaining a deeper insight about anticancer activities of fulvic acid. In this study, the mechanism of interaction between fulvic acid and Tf, has been investigated by using fluorescence quenching, thermodynamics, synchronous fluorescence and circular dichroism (CD) under physiological condition. Our results have shown that fulvic acid binds to Tf and form a new complex, and the calculated apparent association constants are 5.04 x 10(8) M-1, 5.48 x 10(7) M-1, 7.38 x 10(6) M-1 from the fluorescence quenching at 288 K, 298 K, and 310 K. The thermodynamic parameters indicate that hydrogen bonding and weak van der Waals are involved in the interaction between fulvic acid and Tf. The binding of fulvic acid to Tf causes the alpha-helix structure content of the protein to reduce, and resulting that peptide chains of Tf become more stretched. Our results have indicated a mechanism of the interaction between fulvic acid and Tf, which may provide information for possible design of methods to deliver drug molecules via transferrin to target tissues and cells effectively. (C) 2014 Elsevier B.V. All rights reserved.
机译：转铁蛋白已显示出将抗癌药递送至过度表达转铁蛋白受体的主要增殖癌细胞中的潜力。黄腐酸具有广泛的生物学和药理活性，引起广泛关注，黄腐酸与人血清转铁蛋白（Tf）的相互作用对于深入了解黄腐酸的抗癌活性具有重要意义。在这项研究中，通过在生理条件下使用荧光猝灭，热力学，同步荧光和圆二色性（CD）研究了富里酸与Tf之间的相互作用机理。我们的结果表明，富里酸与Tf结合并形成新的络合物，计算出的表观缔合常数为5.04 x 10（8）M-1、5.48 x 10（7）M-1、7.38 x 10（6）M -1在288 K，298 K和310 K处的荧光猝灭产生-1。热力学参数表明氢键和弱范德华力参与了富里酸和Tf的相互作用。黄腐酸与Tf的结合导致蛋白质的α-螺旋结构含量降低，并导致Tf的肽链变得更拉伸。我们的研究结果表明了富里酸和Tf之间相互作用的机制，这可能为通过转铁蛋白有效地将药物分子传递至靶组织和细胞的方法设计提供信息。 （C）2014 Elsevier B.V.保留所有权利。

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