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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Investigation of interaction studies of cefpirome with ACE-inhibitors in various buffers
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Investigation of interaction studies of cefpirome with ACE-inhibitors in various buffers

机译：在各种缓冲液中头孢哌啶与ACE抑制剂相互作用的研究

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This work describes a RP-HPLC method for the determination and interaction studies of cefpirome with ACE-inhibitors (captopril, enalapril and lisinopril) in various buffers. The separation and interaction of cefpirome with ACE-inhibitors was achieved on a Purospher Star, C-18 (5 mu m, 250 x 4.6 mm) column. Mobile phase consisted of methanol: water (80:20, v/v, pH 3.3); however, for the separation of lisinopril, it was modified to methanol-water (40:60, v/v, pH 3.3) and pumped at a flow rate of 1 mL(-1) min. In all cases, UV detection was performed at 225 nm. Interactions were carried out in physiological pH i.e., pH 1 (simulated gastric juice), 4 (simulated full stomach), 7.4 (blood pH) and 9 (simulated GI), drug contents were analyzed by reverse phase high performance liquid chromatography. Method was found linear in the concentration range of 1.0-50.0 mu g mL(-1) with correlation coefficient (r(2)) of 0.999. Precision (RSD%) was less than 2.0%, indicating good precision of the method and accuracy was 98.0-100.0%. Furthermore, cefpirome-ACE-inhibitors' complexes were also synthesized and results were elucidated on the basis of FT-IR, and H-1 NMR. The interaction results show that these interactions are pH dependent and for the co-administration of cefpirome and ACE-inhibitors, a proper interval should be given. (C) 2014 Elsevier B.V. All rights reserved.
机译：这项工作描述了一种RP-HPLC方法，用于测定各种缓冲液中头孢哌酮与ACE抑制剂（卡托普利，依那普利和赖诺普利）的相互作用。在Purospher Star，C-18（5μm，250 x 4.6 mm）色谱柱上实现头孢哌酮与ACE抑制剂的分离和相互作用。流动相由甲醇：水（80：20，v / v，pH 3.3）组成;但是，为了分离赖诺普利，将其改性为甲醇-水（40：60，v / v，pH 3.3）并以1 mL（-1）min的流速泵送。在所有情况下，均在225 nm处进行UV检测。在生理pH，即pH 1（模拟胃液），4（模拟全胃），7.4（血液pH）和9（模拟GI）中进行相互作用，通过反相高效液相色谱法分析药物含量。方法在1.0-50.0μg mL（-1）的浓度范围内呈线性，相关系数（r（2））为0.999。精密度（RSD％）小于2.0％，表明该方法的精密度较高，准确度为98.0-100.0％。此外，还合成了头孢哌啶-ACE抑制剂的复合物，并基于FT-IR和H-1 NMR阐明了结果。相互作用结果表明，这些相互作用是pH依赖性的，对于头孢哌酮和ACE抑制剂的共同给药，应给出适当的间隔。 （C）2014 Elsevier B.V.保留所有权利。

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