Deep-UV Laser-Based Fluorescence Lifetime Imaging Microscopy of Single Molecules

Qiang Li; Thomas Ruckstuhl; Stefan Seeger

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Deep-UV Laser-Based Fluorescence Lifetime Imaging Microscopy of Single Molecules

机译：基于深紫外激光的单分子荧光寿命成像显微镜

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Deep ultraviolet (deep-UV) laser-based fluorescence lifetime imaging microscopy of single dye molecules adsorbed on quartz glass surfaces is presented.The instrumental setup uses a mode-locked diode-pumped picosecond laser emitting at 266 nm with a repetition rate of 40 MHz.Applying the time-resolved single-photon counting method,we investigated the laser dye 2,2'"-dimethyl-p-quaterphenyl and obtained the fluorescence intensity and fluorescence lifetime images of individual adsorbed molecules.The described setup is also well-suited for biological applications for ultrasensitive detection of intrinsic fluorescence after UV excitation.

机译：提出了基于深紫外（deep-UV）激光的荧光寿命成像显微镜，用于吸附在石英玻璃表面的单个染料分子。该仪器设置使用锁模二极管泵浦皮秒激光，在266 nm处发射，重复频率为40 MHz 。采用时间分辨单光子计数方法，我们研究了激光染料2,2'“-二甲基-对-四季苯基，并获得了单个吸附分子的荧光强度和荧光寿命图像。所描述的设置也非常适合用于紫外线激发后超灵敏检测内在荧光的生物学应用。

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《The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical》 |2004年第24期|共6页
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Qiang Li; Thomas Ruckstuhl; Stefan Seeger;
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1. Deep-UV Laser-Based Fluorescence Lifetime Imaging Microscopy of Single Molecules [J] . Qiang Li, Thomas Ruckstuhl, Stefan Seeger The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical . 2004,第24期

机译：基于深紫外激光的单分子荧光寿命成像显微镜
2. Single-molecule Imaging and fluorescence lifetime imaging microscopy show different structures for high-and low-affinity epidermal growth factor receptors in A431 cells [J] . Webb SED, Roberts SK, Needham SR, Biophysical Journal . 2008,第3期

机译：单分子成像和荧光寿命成像显微镜显示A431细胞中高亲和力和低亲和力表皮生长因子受体的结构不同
3. Spectrally resolved fluorescence lifetime imaging microscopy (SFLIM)—an appropriate method for imaging single molecules in living cells [J] . Jens-Peter Knemeyer, Nicole Marmé, J?rg D. Hoheisel Analytical and Bioanalytical Chemistry . 2007,第1期

机译：光谱解析荧光寿命成像显微镜（SFLIM）-活细胞中单个分子成像的合适方法
4. Counting single molecules in living cells at high resolution by spectrally resolved fluorescence lifetime imaging microscopy (SFLIM) and coincidence analysis [C] . T. Heinlein, P. Schlueter, D.P. Herten, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; Progress in Biomedical Optics and Imaging; vol.6 no.14 . 2005

机译：通过光谱解析荧光寿命成像显微镜（SFLIM）和重合分析以高分辨率对活细胞中的单分子进行计数
5. Fluorescence lifetime-resolved imaging microscopy studies: Quantitative image analysis, spectral-flim, and photosynthesis. [D] . Chen, Yi-Chun. 2010

机译：荧光寿命分辨成像显微镜研究：定量图像分析，光谱平移和光合作用。
6. Single-Molecule Imaging and Fluorescence Lifetime Imaging Microscopy Show Different Structures for High- and Low-Affinity Epidermal Growth Factor Receptors in A431 Cells [O] . Stephen E.D. Webb, Selene K. Roberts, Sarah R. Needham, 2008

机译：单分子成像和荧光寿命成像显微镜显示高和低亲和力表皮生长因子受体在A431细胞中的不同结构。
7. Single-Molecule Imaging and Fluorescence Lifetime Imaging Microscopy Show Different Structures for High- and Low-Affinity Epidermal Growth Factor Receptors in A431 Cells☆ [O] . Webb, Stephen E.D., Roberts, Selene K., Needham, Sarah R., 2008

机译：单分子成像和荧光寿命成像显微镜显示A431细胞中高亲和力和低亲和力表皮生长因子受体的结构不同☆

Deep-UV Laser-Based Fluorescence Lifetime Imaging Microscopy of Single Molecules

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