...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Fluorescence Correlation Spectroscopy at Micromolar Concentrations without Optical Nanoconfinement
【24h】

Fluorescence Correlation Spectroscopy at Micromolar Concentrations without Optical Nanoconfinement

机译：没有光学纳米约束的微摩尔浓度的荧光相关光谱

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fluorescence correlation spectroscopy (FCS) is an important technique for studying biochemical interactions dynamically that may be used in vitro and in cell-based studies. It is generally claimed that FCS may only be used at nM concentrations. We show that this general consensus is incorrect and that the limitation to nM concentrations is not fundamental but due to detector limits as well as laser fluctuations. With a high count rate detector system and applying laser fluctuation corrections, we demonstrate FCS measurements up to 38 μM with the same signal-to-noise as at lower concentrations. Optical nanoconfinement approaches previously used to increase the concentration range of FCS are not necessary, and further increases above 38 μM may be expected using detectors and detector arrays with higher saturation rates and better laser fluctuation corrections. This approach greatly widens the possibilities of dynamic measurements of biochemical interactions using FCS at physiological concentrations.
机译：荧光相关光谱法（FCS）是动态研究生化相互作用的一项重要技术，可在体外和基于细胞的研究中使用。通常要求FCS只能以nM浓度使用。我们表明，这种普遍共识是不正确的，并且对nM浓度的限制不是根本性的，而是由于探测器的限制以及激光的波动。使用高计数率检测器系统并应用激光波动校正，我们证明了与低浓度下相同的信噪比，FCS测量高达38μM。以前没有必要使用光学纳米约束方法来增加FCS的浓度范围，并且可以使用具有更高饱和率和更好的激光波动校正的探测器和探测器阵列，将其进一步提高到38μM以上。这种方法极大地拓宽了使用生理浓度的FCS动态测量生化相互作用的可能性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号