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首页> 外文期刊>Journal of Applied Polymer Science >Application of chitosan-entrapped beta-galactosidase in a packed-bed reactor system
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Application of chitosan-entrapped beta-galactosidase in a packed-bed reactor system

机译：壳聚糖包埋的β-半乳糖苷酶在填充床反应器系统中的应用

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The model enzyme beta-galactosidase was entrapped in chitosan gel beads and tested for hydrolytic activity and its potential for application in a packed-bed reactor. The chitosan beads had an enzyme entrapment efficiency of 59% and retained 56% of the enzyme activity of the free enzyme. The Michaelis constant (K-m) was 0.0086 and 0.011 mumol/mL for the free and immobilized enzymes, respectively. The maximum velocity of the reaction (V-max) was 285.7 and 55.25 mumol mL(-1) min(-1) for the free and immobilized enzymes, respectively. In pH stability tests, the immobilized enzyme exhibited a greater range of pH stability and shifted to include a more acidic pH optimum, compared to that of the free enzyme. A 2.54 X 16.51-cm. tubular reactor was constructed to hold 300 mL of chitosan-immobilized enzyme. A full-factorial test design was implemented to test the effect of substrate flow (20 and 100 mL/min), concentration (0.0015 and 0.003M), and repeated use of the test bed on efficiency of the system. Parameters were analyzed using repeated-measures analysis of variance. Flow (p < 0.05) and concentration (p < 0.05) significantly affected substrate conversion, as did the interaction progressing from Run 1 to Run 2 on a bed (p < 0.05). Reactor stability tests indicated that the packed-bed reactor continued to convert substrate for more than 12 h with a minimal reduction in conversion efficiency. (C) 2003 Wiley Periodicals, Inc. [References: 23]
机译：将模型酶β-半乳糖苷酶截留在壳聚糖凝胶珠中，并测试其水解活性及其在填充床反应器中的应用潜力。壳聚糖珠粒的酶截留率为59％，并保留了游离酶的56％的酶活性。游离酶和固定酶的米氏常数（K-m）分别为0.0086和0.011μmol/ mL。游离和固定化酶的最大反应速度（V-max）分别为285.7和55.25 mumol mL（-1）min（-1）。在pH稳定性测试中，与游离酶相比，固定化酶表现出更大范围的pH稳定性，并且转移到包括更酸性的最佳pH值。 2.54 X 16.51厘米建造管状反应器以容纳300 mL固定有壳聚糖的酶。实施了全要素测试设计，以测试底物流量（20和100 mL / min），浓度（0.0015和0.003M）以及重复使用测试床对系统效率的影响。使用方差的重复测量分析来分析参数。流量（p <0.05）和浓度（p <0.05）显着影响底物转化率，从床上的运行1到运行2的相互作用也是如此（p <0.05）。反应器稳定性测试表明，填充床反应器继续将底物转化超过12小时，而转化效率的降低最小。 （C）2003 Wiley Periodicals，Inc. [参考：23]

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