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首页> 外文期刊>Journal of Applied Polymer Science >Development of an amperometric biosensor based on a redox-mediator-doped polypyrrole film
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Development of an amperometric biosensor based on a redox-mediator-doped polypyrrole film

机译：基于氧化还原介质掺杂的聚吡咯膜的电流型生物传感器的开发

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An amperometric biosensor was developed for the quantitative estimation of phenolic compounds in aqueous media. The enzyme tyrosinase [poly(phenol oxidase) (PPO)] was adsorbed onto a hexacyanoferrate(II)-ion-doped conducting polypyrrole (PPY) film deposited on an indium tin oxide (ITO) coated glass-plate support. The PPO activity in the PPO/Fe2+-PPY/ITO film was assayed as a function of the concentration of phenolic compounds. Cyclic voltammetric studies were carried out on this enzyme electrode, and the surface morphology of the enzyme-immobilized polymer film was studied with scanning electron microscopy. The results of the amperometric response of the PPO/Fe2+-PPY/ITO film showed sensitivities of 0.14, 0.21, and 0.36 A M(-1)cm(-2) and linear response ranges of 9.9-84.7, 6.7-72.6, and 3.9-48.8 muM for phenol, catechol, and p-chlorophenol, respectively. The PPO/Fe2+-PPY/ITO electrode exhibited a response time of about 50 s and was stable for about 12 weeks at 4degreesC. (C) 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 927-933, 2004.
机译：安培生物传感器被开发用于定量估计水性介质中的酚类化合物。将酪氨酸酶[聚（酚氧化酶）（PPO）]吸附到沉积在涂有氧化铟锡（ITO）的玻璃板载体上的六氰合铁酸酯（II）离子掺杂的导电聚吡咯（PPY）膜上。测定PPO / Fe2 + -PPY / ITO膜中的PPO活性与酚类化合物浓度的关系。在该酶电极上进行循环伏安法研究，并通过扫描电子显微镜研究酶固定的聚合物膜的表面形态。 PPO / Fe2 + -PPY / ITO膜的安培响应结果显示灵敏度为0.14、0.21和0.36 AM（-1）cm（-2），线性响应范围为9.9-84.7、6.7-72.6和3.9苯酚，邻苯二酚和对氯苯酚分别为-48.8μM。 PPO / Fe2 + -PPY / ITO电极显示约50 s的响应时间，并在4℃稳定约12周。 （C）2004 Wiley Periodicals，Inc. J Appl Polym Sci 93：927-933，2004。

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