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首页> 外文期刊>Journal of Applied Polymer Science >Amorphous Poly(3-hydroxybutyrate) Nanoparticles Prepared with Recombinant Phasins and PHB Depolymerase
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Amorphous Poly(3-hydroxybutyrate) Nanoparticles Prepared with Recombinant Phasins and PHB Depolymerase

机译：用重组蛋白和PHB解聚酶制备的非晶态聚3-羟基丁酸酯纳米粒子

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摘要

Phasin protein (PhaP) is known to anchor into the matrix of phospholipid surrounding polyhydroxyalkanoic acid (PHA) inclusion bodies formed in bacterial cells and regulate the size of the granules, as well as the number of PHA granules. To investigate the effect of phasin on the formation of artificial poly(3-hydroxybutyrate) (P(3HB)) granules in vitro, (His)_6-tagged or GST-fusion recombinant phasin was prepared and utilized for the artificial granule preparation. In addition, a P(3HB) depolymerase was coloaded with the recombinant phasin to prepare self-degradable phasin-coated P(3HB) granules. A water/chloroform two-phase emulsion technique was used, in which the emulsification was carried out by sonication, and the chloroform in the emulsion was removed by stirring-aided evaporation at room temperature or 65°C. Slower chloroform removal at room temperature produced better spherically shaped P(3HB) nanogranules, which were uniformly sized (~100 to 200 nm in diameter). The self-degradability of P(3HB) depolymerase-loaded P(3HB) nanogranules was investigated.
机译：已知蛋白蛋白（PhaP）可以锚定在细菌细胞中形成的聚羟基链烷酸（PHA）包涵体周围的磷脂基质中，并调节颗粒的大小以及PHA颗粒的数量。为了研究phasin在体外对人工聚（3-羟基丁酸酯）（P（3HB））颗粒形成的影响，制备了（His）-6标签或GST融合的重组phasin，并用于制备人工颗粒。此外，将P（3HB）解聚酶与重组phasin一起负载，以制备可自降解的phasin涂层P（3HB）颗粒。使用水/氯仿两相乳液技术，其中通过超声处理进行乳化，并在室温或65℃下通过搅拌辅助蒸发除去乳液中的氯仿。在室温下较慢的氯仿去除速度会产生更好的球形P（3HB）纳米颗粒，其大小均一（直径约100至200 nm）。研究了P（3HB）解聚酶负载的P（3HB）纳米颗粒的自降解性。

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