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首页> 外文期刊>Journal of Materials Science >Study on the structure of SF fiber mats electrospun with HFIP and FA and cells behavior
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Study on the structure of SF fiber mats electrospun with HFIP and FA and cells behavior

机译：HFIP和FA电纺SF纤维毡的结构及细胞行为的研究。

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Bombyx mori silk fibroin (SF) fiber mats were prepared by electrospinning with the solvent of hexafluoroisopropanol (HFIP) and formic acid (FA). The average diameters of SF fiber mats observed by SEM were 2.0 and 0.3 mu m when different solvent, HFIP and FA, were used. Fourier transform infrared and X-ray diffraction were employed to study the secondary structure of the SF fiber mats; the results showed that the electrospin solvent not only affect the secondary structure of as-spun SF fiber mats, but also indirectly affect the structure transition of SF fiber mats post-treatment with ethanol. And the SF fiber mats electrospun with FA showed more beta-sheet structure before and after ethanol treatment. The differential thermal analysis curve indicated that the solvent of HFIP or FA had a weak effect on the thermal properties of SF fiber mats. To assay the cytocompatibility and cell behavior on the SF fiber mats, cell attachment, spreading, and proliferation of normal human epidermal fibroblasts (NHEF) seeded on the scaffolds was studied. The results indicated that the SF fiber mats support NHEF attachment and growth on SF fiber mats in vitro, and no difference between the SF fiber mats electrospun with HFIP and FA was observed. In this article, a desired morphology and secondary structure of SF fiber mats could be prepared by choosing different electrospinning solvent.
机译：家蚕丝素蛋白（SF）纤维垫是通过用六氟异丙醇（HFIP）和甲酸（FA）进行电纺丝而制备的。当使用不同的溶剂HFIP和FA时，通过SEM观察到的SF纤维毡的平均直径为2.0和0.3μm。用傅里叶变换红外和X射线衍射研究了SF纤维毡的二级结构。结果表明，静电纺丝溶剂不仅影响纺后的SF纤维毡的二级结构，而且还间接影响了乙醇处理后SF纤维毡的结构转变。 SF处理的SF纤维毡在乙醇处理前后显示出更多的β-折叠结构。差示热分析曲线表明，HFIP或FA的溶剂对SF纤维毡的热性能影响较小。为了测定SF纤维垫上的细胞相容性和细胞行为，研究了接种在支架上的正常人表皮成纤维细胞（NHEF）的细胞黏附，扩散和增殖。结果表明，SF纤维毡在体外支持NHEF附着并在SF纤维毡上生长，并且用HFIP和FA静电纺制的SF纤维毡之间没有差异。在本文中，可以通过选择不同的电纺丝溶剂来制备所需的SF纤维毡形态和二级结构。

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