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首页> 外文期刊>Journal of Molecular Biology >One is not enough.
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One is not enough.

机译：一个还不够。

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摘要

In both prokaryotic and eukaryotic organisms, repressors and activators are responsible for regulating gene expression. The lac operon is a paradigm for understanding how metabolites function as signaling molecules and modulate transcription. These metabolites or allosteric effector molecules bind to the repressor and alter the conformational equilibrium between the induced and the repressed states. Here, we describe a set of experiments where we modified a single inducer binding site in a dimeric repressor and examined its effect on induction. Based upon these observations, we have been able to calculate the thermodynamic parameters that are responsible for the allosteric properties that govern repressor function. Understanding how effector molecules alter the thermodynamic properties of the repressor is essential for establishing a detailed understanding of gene regulation.
机译：在原核和真核生物中，阻遏物和激活物负责调节基因表达。紫胶操纵子是理解代谢物如何充当信号分子和调节转录的范例。这些代谢物或变构效应分子与阻遏物结合，并改变诱导状态和受阻状态之间的构象平衡。在这里，我们描述了一组实验，其中我们修饰了二聚阻遏物中的单个诱导物结合位点，并检查了其对诱导的影响。基于这些观察，我们已经能够计算出热力学参数，这些参数负责控制阻遏物功能的变构性质。了解效应子分子如何改变阻遏物的热力学性质对于建立对基因调控的详细了解至关重要。

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