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首页> 外文期刊>Life sciences >Cholinergic receptors in the murine oviduct: inventory and coupling to intracellular calcium concentration.
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Cholinergic receptors in the murine oviduct: inventory and coupling to intracellular calcium concentration.

机译：小鼠输卵管中的Cholinergic受体：库存和偶联与细胞内钙浓度。

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In the oviduct, muscarinic acetylcholine receptors (MR) are linked with motility regulation and nicotinic receptors (nAChR) with ectopic pregnancy. We here aimed to determine the repertoire of cholinergic receptor expression in the murine oviduct and their functional coupling to regulation of intracellular calcium concentration ([Ca(2+)](i)).Cholinergic receptor transcripts were assessed by RT-PCR in oviductal segments (ampulla, isthmus, uterotubar junction) in all cyclic stages and pregnancy, and in laser-microdissected samples of epithelium and smooth muscle, nAChR subunit α3 distribution in tissue sections using an appropriate genetic reporter mouse strain. [Ca(2+)](i) responses were monitored in ciliated and non-ciliated oviductal cells isolated from wild-type and MR subtypes 1 and 3 gene deficient mice.Transcripts for all MR subtypes (M1-M5) are constantly expressed whereas there is some variability in nAChR expression from individual to individual. The qualitative expression pattern is independent from the hormonal status of the animal, except for nAChR α7, which is less present during pregnancy. The epithelium expresses M1, M3, nAChR α7 (data from laser-assisted microdissection) and nAChR α3 (ultrastructural investigation of reporter mice). MR dominate over nAChR in increasing [Ca(2+)](i) with being M3 the major, but not sole subtype driving this effect. The general nAChR inhibitor mecamylamine enhances muscarinic and purinergic responses.In conclusion, the murine oviduct is endowed with a multiplicity of muscarinic and nicotinic receptors subtypes that, with respect to regulation of [Ca(2+)](i), are inversely linked to each other. The major, but not sole, cholinergic receptor driving increase in [Ca(2+)](i) is M3.
机译：在输卵管中，肌肉素乙酰胆碱受体（MR）与胞外妊娠的运动性调节和烟碱受体（NACHR）连接。我们在这里旨在确定鼠输卵管中的胆碱能受体表达的曲目及其与细胞内钙浓度调节的功能偶联（[Ca（2 +）]（I））。通过RO-PCR在输卵管段中评估胆碱能受体转录物（Ampulla，isthmus，utertotubar结）在所有循环阶段和妊娠中，以及激光微小的上皮和平滑肌样品，Nachr亚基α3在组织切片中的分布使用适当的遗传报告小鼠菌株。 [Ca（2 +）]（i）在纤维化和非纤维的流动细胞中监测从野生型和MR亚型1和3基因缺乏小鼠中分离的非纤维的卵体细胞中的反应。对于所有MR亚型（M1-M5）的手术名称不断表达，而是从个人到个人的NACHR表达有一些可变性。定性表达模式与动物的荷尔蒙状态无关，除了在怀孕期间较低的NACHRα7。上皮表达M1，M3，NACHRα7（来自激光辅助微量微小的数据）和NACHRα3（报告小鼠的超微结构调查）。先生在NACHR上占据了[CA（2 +）]（i）的主要，但不是唯一的亚型驱动这种效果。一般的NACHR抑制剂MeCamylamine增强了肌肉蛋白和嘌呤能反应。结论，小鼠输卵管赋予多种毒蕈碱和烟碱受体亚型，即关于[Ca（2 +）]（I）的调节，与之相反彼此。主要，但不是唯一的胆碱能受体驱动增加[Ca（2 +）]（i）是m3。

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